PMID: 11330830May 2, 2001Paper

Evaluation of antioxidant activity of epigallocatechin gallate in biphasic model systems in vitro

Molecular and Cellular Biochemistry
C Hu, D D Kitts

Abstract

The antioxidant activity of epigallocatechin gallate (EGCG) was studied in different in vitro model systems, which enabled evaluation of both chemical and physical factors involved in assessing the role of EGCG in oxidative reactions. EGCG suppressed the initiation rate and prolonged the lag phase duration of peroxyl radical-induced oxidation in a phospholipid liposome model to a greater extent (p < 0.01) compared to both Trolox and alpha-tocopherol. Effectiveness of these antioxidants to prolong the peroxyl radical-induced lag phase was inversely related to lipophilic character. EGCG also protected against both peroxyl radical and hydroxyl radical-induced supercoiled DNA nicking. The rate constant describing EGCG reaction against hydroxyl radical was 4.22+/-0.07 x 10(10) M(-1) x sec(-1), which was comparable to those of Trolox and alpha-tocopherol, respectively. EGCG exhibited a synergistic effect with alpha-tocopherol in scavenging 1,1-diphenyl-2-picylhydrazyl (DPPH) radical, thus displaying a direct free radical scavenging capacity. In vitro Cu2+-induced-human LDL oxidation was accelerated in the presence of EGCG and attributed to the conversion of Cu2+ to Cu+. We conclude that the particularly effective antioxidant properti...Continue Reading

Citations

May 18, 2016·Journal of Immunology Research·Jin-Biao LiuJie-Liang Li
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Agapios SachinidisJürgen Fingerle
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