Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model

Scientific Reports
Jessica M MotherwellWalter L Murfee

Abstract

An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in angiogenesis within an intact microvascular network using time-lapse imaging. A critical question remains whether the vessels maintain their functionality. The objective of this study was to determine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to constrict. Adult rat mesenteric tissues were harvested and cultured for three days in either MEM or MEM plus 10% serum. On Day 0 and Day 3 live microvascular networks were visualized with FITC conjugated BSI-lectin labeling and arteriole diameters were compared before and five minutes after topical exposure to vasoconstrictors (50 mM KCl and 20 nM Endothelin-1). Arterioles displayed a vasoconstriction response to KCl and endothelin for each experimental group. However, the Day 3 serum cultured networks were angiogenic, characterized by increased vessel density, and displayed a decreased vasoconstriction response compared to Day 0 networks. T...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Jul 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·O SagherK S Lee
Jun 1, 1996·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·T C Skalak, R J Price
Mar 31, 2000·Nature Medicine·P Carmeliet
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·L PelletierP Charbord
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·D SunG Kaley
Jan 22, 2003·Microvascular Research·Ana M Ponce, Richard J Price
Jul 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Christopher R AndersonRichard J Price
Dec 3, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Prasad V G KatakamDavid W Busija
Apr 22, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter L MurfeeThomas C Skalak
Sep 23, 2006·Journal of Cellular and Molecular Medicine·K Norrby
May 23, 2008·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Xiao-Chen RuQiang Xia
Oct 10, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wensheng Chen, Raouf A Khalil
Oct 5, 2010·Nature Protocols·Suphansa SawamiphakAmparo Acker-Palmer
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jonathan W Song, Lance L Munn
Sep 8, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Michael J Davis
Oct 1, 2011·Microvascular Research·Peter C Stapor, Walter L Murfee
May 31, 2012·Journal of Visualized Experiments : JoVE·Ming YangWalter L Murfee
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 9, 2012·Circulation Research·Simon BulleyJonathan H Jaggar
Nov 6, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Peter C StaporWalter L Murfee
Jan 17, 2013·Tissue Engineering. Part C, Methods·Monica L MoyaSteven C George
Mar 25, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Richard S SweatWalter L Murfee
Mar 10, 2016·Pharmacological Reviews·Anthony P DavenportJanet J Maguire

❮ Previous
Next ❯

Citations

Apr 13, 2018·Journal of Applied Physiology·Nicholas A HodgesWalter L Murfee
May 21, 2018·Journal of Vascular Research·Ariana D Suarez-MartinezWalter L Murfee
Sep 5, 2018·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Ariana D Suarez-MartinezWalter L Murfee
Apr 14, 2018·Scientific Reports·Jessica M MotherwellWalter L Murfee
Oct 5, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Mohammad S AzimiWalter L Murfee
Jul 10, 2019·Tissue Engineering. Part C, Methods·Jessica M MotherwellWalter L Murfee
Sep 6, 2020·Journal of Neuroscience Methods·Nicholas A HodgesWalter L Murfee

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

Prism
SigmaStat
NIH Fiji
excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Microcirculation : the Official Journal of the Microcirculatory Society, Inc
Richard S SweatWalter L Murfee
Integrative Biology : Quantitative Biosciences From Nano to Macro
Theresa B PhamduyDouglas B Chrisey
© 2022 Meta ULC. All rights reserved