Evaluation of ATR-FTIR for analysis of bacterial cellulose impurities

Journal of Microbiological Methods
Mark E FullerKevin McClay

Abstract

This research evaluated the utility of using the large amount of spectral data obtained during attenuated total reflection Fourier-transform infrared spectrophotometry (ATR-FTIR) analysis of dried biocellulose (BC) to estimate the type and concentration of potential bacterial cells impurities present in the BC. Pre-cleaned BC was contaminated with known concentrations of representative nucleic acid, lipid, and protein impurities, as well as whole bacterial cells. These impurity standards were then subjected to ATR-FTIR analysis, and the resulting spectral data were used to develop models to estimate the concentrations of impurities in differentially processed BC. Results indicated that ATR-FTIR is a useful tool for estimating impurities in BC, and may also be applicable for measurement of levels of non-cellulose biomolecules added to BC for various purposes.

References

Jan 1, 1990·Applied Biochemistry and Biotechnology·J D FontanaL F Farah
Jun 2, 2011·Journal of Industrial Microbiology & Biotechnology·Xiaobo ZengHaiying Wang
Nov 28, 2013·Carbohydrate Polymers·Sherif M A S Keshk
Feb 11, 2015·Applied Microbiology and Biotechnology·J M RajwadeJ V Kumbhar

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Citations

May 16, 2019·Microbial Biotechnology·Patricia MarínSilvia Marqués
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Georgiana-Mădălina Lemnaru PopaLia-Mara Dițu
Jun 29, 2021·Cellulose·Henrique Augusto Silva ValentinoTalita M Lacerda
Sep 25, 2020·Environmental Science & Technology·Marcus C HollandKatherine R Zodrow

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