Evaluation of automated loop-mediated amplification (LAMP) for routine malaria detection in blood samples of German travelers - A cross-sectional study

Travel Medicine and Infectious Disease
Hagen FrickmannEgbert Tannich

Abstract

We assessed a commercial loop-mediated amplification (LAMP) platform for its reliability as a screening tool for malaria parasite detection. A total of 1000 blood samples from patients with suspected or confirmed malaria submitted to the German National Reference Center for Tropical Pathogens were subjected to LAMP using the Meridian illumigene Malaria platform. Results were compared with microscopy from thick and thin blood films in all cases. In case of discordant results between LAMP and microscopy (n = 60), confirmation testing was performed with real-time PCR. Persistence of circulating parasite DNA was analyzed by serial assessments of blood samples following malaria treatment. Out of 1000 blood samples analyzed, 238 were positive for malaria parasites according to microscopy (n = 181/1000) or PCR (additional n = 57/60). LAMP demonstrated sensitivity of 98.7% (235/238), specificity of 99.6% (759/762), positive predictive value (PPV) of 98.7% (235/238) and negative predictive value (NPV) of 99.6% (759/762), respectively. For first slides of patients with malaria and for follow-up slides, sensitivity values were 99.1% (106/107) and 98.5% (129/131), respectively. The performance of the Meridian illumigene Malaria platform is...Continue Reading

Citations

Jan 25, 2019·The American Journal of Tropical Medicine and Hygiene·Gitte N HartmeyerMichael Kemp
May 30, 2019·Acta Microbiologica Et Immunologica Hungarica·Philipp A ReukenJürgen Rödel
Feb 6, 2019·European Journal of Microbiology & Immunology·Hans KollendaHagen Frickmann
Mar 13, 2020·Clinical Microbiology Reviews·Johannes MischlingerMichael Ramharter
Oct 14, 2020·Malaria Journal·Melina HeinemannThierry Rolling
Dec 8, 2020·American Journal of Clinical Pathology·Eric McGinnisKristine Roland
Jul 6, 2021·Frontiers in Cellular and Infection Microbiology·Alba Marina GimenezDaniel Youssef Bargieri
Jul 14, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Stephane PicotAnne-Lise Bienvenu

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