Evaluation of benzimidazole resistance status in Ascaridia galli

Parasitology
B TarbiatJ Höglund

Abstract

Susceptability of Ascaridia galli to benzimidazole (BZ) was investigated using faecal egg count reduction test (FECRT), in ovo larval development test (LDT) and genetic markers (mutations at codons 167, 198 and 200 of β-tubulin gene). Six flocks (F1-F6) of a commercial laying hen farm with different number of exposure to BZ were recruited. The FECR was calculated by analyzing individual faeces (F1, F2, F4 and F5) before and 10 days after treatment. The LDT was performed on parasite eggs from pooled samples from F1 to F6 and LC50 and LC99 were calculated. DNA was extracted from 120 worms and sequenced for β-tubulin gene. In all flocks, the FECRs were above 95% (lower CI above 90%). No significant difference was observed (p > 0·05) among obtained LC50 (F1/F4 and F2/F5 vs F3/F6) in the LDT. However, LC50 and LC99 were higher than suggested values for declaration of resistance in other nematode species. No variation was observed in codon positions involved in BZ resistance. Overall, our results indicated lack of evidence of resistance to BZ in A. galli. More research is needed to confirm these results and to further optimize the existing tools for detection and monitoring of anthelmintic resistance in A. galli.

References

Apr 1, 1995·International Journal for Parasitology·J H GillE Lacey
Aug 1, 1994·Veterinary Parasitology·R Prichard
May 22, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·L T WierJ P Foreyt
Mar 19, 2002·Molecular and Biochemical Parasitology·Anne Silvestre, Jacques Cabaret
Oct 22, 2002·Veterinary Research·Gerald C Coles
Oct 22, 2002·Veterinary Research·Ray M Kaplan
Oct 2, 2003·Veterinary Parasitology·T A YazwinskiUNKNOWN World Association for the Advancement of Veterinary Parasitology
Jun 16, 2004·Current Opinion in Cell Biology·Douglas N Robinson, James A Spudich
Nov 27, 2004·Research in Veterinary Science·G C Coles
Jan 24, 2006·Veterinary Parasitology·G C ColesJ Vercruysse
Aug 18, 2006·New Zealand Veterinary Journal·P B McKenna
Dec 17, 2008·Journal of the American Veterinary Medical Association·Sue B HowellRay M Kaplan
Jun 23, 2009·Trends in Parasitology·Catherine E JamesMary W Davey
Dec 14, 2011·Veterinary Parasitology·Ray M Kaplan, Anand N Vidyashankar
May 21, 2013·Molecular and Biochemical Parasitology·E TydénJ Höglund
Mar 4, 2014·Parasitology Research·Gürbüz Daş, Matthias Gauly
Mar 19, 2014·Veterinary Parasitology·Laura RinaldiGiuseppe Cringoli
Dec 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Andrew C KotzeRoger K Prichard
Feb 28, 2015·Veterinary Parasitology·Behdad TarbiatJohan Höglund

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Citations

Mar 19, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Adalid PalmaGustavo Fontecha
Jan 23, 2020·Avian Diseases·Katherine Lynn Cupo, Robert Byron Beckstead
Sep 2, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Frida MartinEva Tydén

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