Evaluation of binding selectivities and affinities of platinum-based quadruplex interactive complexes by electrospray ionization mass spectrometry.

Biopolymers
Sarah E PierceJennifer S Brodbelt

Abstract

The quadruplex binding affinities and selectivities of two large pi-surface Pt(II) phenanthroimidazole complexes, as well as a smaller pi-surface platinum bipyridine complex and a larger Ru(II) complex, were evaluated by electrospray ionization mass spectrometry. Circular dichroism (CD) spectroscopy was used to determine the structures of various quadruplexes and to study the thermal denaturation of the quadruplexes in the absence and presence of the metal complexes. In addition, chemical probe reactions with glyoxal were used to monitor the changes in the quadruplex conformation because of association with the complexes. The platinum phenanthroimidazole complexes show increased affinity for several of the quadruplexes with elongated loops between guanine repeats. Quadruplexes with shorter loops exhibited insubstantial binding to the transition metal complexes. Similarly binding to duplex and single strand oligonucleotides was low overall. Although the ruthenium-based metal complex showed somewhat enhanced quadruplex binding, the Pt(II) complexes had higher quadruplex affinities and selectivities that are attributed to their square planar geometries. The chemical probe reactions using glyoxal indicated increased reactivity when...Continue Reading

References

Apr 25, 1991·Nature·A M ZahlerD M Prescott
Oct 1, 1996·Nucleic Acids Research·O N JensenD F Barofsky
May 16, 1998·Biochemistry·T M FletcherL H Hurley
Dec 10, 1998·Nature Medicine·J L Mergny, C Hélène
Mar 31, 2000·Trends in Pharmacological Sciences·H Han, L H Hurley
Jul 13, 2000·The Analyst·P Iannitti-TitoM M Sheil
Oct 24, 2001·Inorganic Chemistry·Matteo CusumanoAntonino Giannetto
Nov 20, 2001·Chemical Reviews·S A Hofstadler, R H Griffey
Sep 11, 2002·Rapid Communications in Mass Spectrometry : RCM·Frédéric RosuEdwin De Pauw
Dec 5, 2002·Chembiochem : a European Journal of Chemical Biology·Carolina CarrascoChristian Bailly
Jul 24, 2003·Chemical Society Reviews·Clive Metcalfe, Jim A Thomas
Dec 3, 2003·Chemical Communications : Chem Comm·Frédéric RosuEdwin De Pauw
Apr 2, 2004·The Journal of Biological Chemistry·Charlotte L Hanson, Carol V Robinson
Oct 30, 2004·Dalton Transactions : an International Journal of Inorganic Chemistry·Thitima UrathamakulStephen F Ralph
Dec 22, 2004·The Journal of Physiology·Anne-Claude GingrasBrian Raught
Jan 11, 2005·The Journal of Antibiotics·Renata L A FurlanGabriel Padilla
Jan 12, 2005·Nucleic Acids Research·Jean-Louis MergnyLaurent Lacroix
Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Satoko AkashiYoshifumi Nishimura
May 24, 2005·Journal of the American Society for Mass Spectrometry·Erin Shammel BakerMichael T Bowers
Nov 4, 2005·Organic & Biomolecular Chemistry·Nicholas M BrownKeith R Fox
Jan 5, 2006·Inorganic Chemistry·Matteo CusumanoAntonino Giannetto
Feb 24, 2006·Journal of the American Chemical Society·Erin Shammel BakerMichael T Bowers
Mar 1, 2006·Journal of the American Society for Mass Spectrometry·Carolyn L MazzitelliSean M Kerwin
Aug 22, 2006·FEBS Letters·Wei LiChun-mei Qiu
Nov 14, 2006·Journal of the American Society for Mass Spectrometry·Carolyn L MazzitelliJennifer S Brodbelt
Apr 4, 2007·Journal of Mass Spectrometry : JMS·Suncerae I SmithJennifer S Brodbelt
May 9, 2007·Biochemical and Biophysical Research Communications·Ilene M PedrosoTerace M Fletcher
May 9, 2007·Rapid Communications in Mass Spectrometry : RCM·Karina C GornallJennifer L Beck
May 19, 2007·Analytical Chemistry·Carolyn L Mazzitelli, Jennifer S Brodbelt
Jun 20, 2007·Nucleic Acids Research·Phillip A RachwalKeith R Fox
Jun 23, 2007·Journal of the American Society for Mass Spectrometry·Carolyn L MazzitelliJennifer S Brodbelt
Aug 28, 2007·Journal of the American Society for Mass Spectrometry·Carolyn L MazzitelliJennifer S Brodbelt
Sep 6, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sarah E EvansVeronika A Szalai
Sep 15, 2007·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Julian Leon Huppert
Nov 9, 2007·Chemical Communications : Chem Comm·Brian M ZeglisJacqueline K Barton

❮ Previous
Next ❯

Citations

Aug 18, 2010·Chemical Communications : Chem Comm·Karina C GornallJennifer L Beck
Jul 20, 2010·Annual Review of Analytical Chemistry·Jennifer S Brodbelt
Sep 13, 2011·Metallomics : Integrated Biometal Science·Tina KamčevaMarijana Petković
May 26, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·S BiancoC Sissi
Mar 13, 2012·International Journal of Biological Macromolecules·Xiaojie CuiGu Yuan
Jan 10, 2012·Journal of Mass Spectrometry : JMS·Silvan R StuckiStefan Schürch
Apr 8, 2011·ChemMedChem·Caterina MusettiClaudia Sissi
May 27, 2010·Angewandte Chemie·Savvas N GeorgiadesRamon Vilar
Jul 3, 2021·Pharmaceuticals·Elisa PalmaAntónio Paulo
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.