PMID: 8950353Nov 4, 1996Paper

Evaluation of blue-chitin column, blue-rayon hanging, and XAD-resin column techniques for concentrating mutagens from two Japanese rivers

Mutation Research
H SakamotoH Hayatsu

Abstract

Highly mutagenic water of the Katsura River, Kyoto, and moderately mutagenic water of the Asahi River, Okayama, were used to evaluate the efficacy of three concentration techniques, the blue-chitin column, the blue-rayon hanging, and the XAD-2 column. These two river waters have been shown to exhibit high mutagenicity in the assay with Salmonella typhimurium TA98 with metabolic activation. With this assay as a measure, two water samples from the Katsura, collected on different dates, and a sample from the Asahi were submitted to the column concentration techniques, blue-chitin and XAD-2. Blue-chitin was more efficient than XAD-2 for all of these samples: e.g., for one Katsura sample, the mutagenicity found was 913 +/- 53 (mean +/- SD, n = 3) revertants/500 ml with blue-chitin, and 419 +/- 129 (n = 3)/500 ml with XAD-2. Blue rayon (0.5 g) hung in the Asahi for 24 h gave 563 +/- 74 (n = 3) revertants, while the water spot-sampled at the start of the hanging showed 253 +/- 10 (n = 3) revertants per 5 liter with the blue-chitin column technique. We conclude that for quantitative measurement of the "Salmonella TA98 +/- S9' mutagens in these rivers, the blue-chitin column is more efficient and accurate than the XAD-2 column and that ...Continue Reading

References

Apr 1, 1977·Mutation Research·T YahagiT Sugimura
Oct 1, 1992·Bulletin of Environmental Contamination and Toxicology·T HashizumeN Kinae
Apr 1, 1990·Bulletin of Environmental Contamination and Toxicology·H Sakamoto, H Hayatsu
Nov 6, 1974·Journal of Chromatography·G A JunkG V Calder
May 1, 1983·Mutation Research·D M Maron, B N Ames
Nov 1, 1994·Mutation Research·W R KusamranT Sugimura
Sep 1, 1993·Archives of Environmental Contamination and Toxicology·T OheM Kawabuti
Apr 6, 1996·Mutation Research·Z RehanaM Ahmad

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Citations

Mar 14, 2003·Environmental Toxicology·Asma Aleem, Abdul Malik
Nov 29, 2002·Journal of Chromatography. a·Joakim BångKerstin Skog
Mar 12, 2013·Mutagenesis·Donna JohnsonRichard M Walmsley
May 12, 2000·Ecotoxicology and Environmental Safety·H EhrlichmannA Eisentraeger
Dec 2, 2004·Mutation Research·Takeshi OheKeiji Wakabayashi
Jun 5, 2004·The Journal of Biological Chemistry·Jing LinDavid J Weber

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