Evaluation of bone marrow stem cell response to PLA scaffolds manufactured by 3D printing and coated with polydopamine and type I collagen

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Bruna Nunes TeixeiraRossana Mara da Silva Moreira Thiré

Abstract

The majority of synthetic polymers used in 3 D printing are not designed to promote specific cellular interactions and hence possess limited bioactivity. Most of the strategies proposed to overcome this limitation demand multiple and expensive processing steps. This study aimed to evaluate the surface modification of 3D-printed poly(lactic acid) (PLA) scaffolds with polydopamine (PDA) coating as an alternative strategy to enhance their bioactivity and to facilitate the immobilization of type I collagen (COL I) onto the implant surface. Physical and chemical properties of PLA scaffolds coated with PDA, COL I or both were evaluated. The response of porcine bone marrow stem cells (MSCs) to the coatings was also investigated. The PDA layer improved COL immobilization onto the surface of the PLA scaffolds by 92%. The combination of PDA and COL functionalizations provided the best conditions for early-stage (<7 days) cell response. In addition, the PDA plus COL surface facilitated the robust deposition of extracellular matrix in the first 14 days of cell culture. Although the behavior of the MSCs appeared to be similar for both uncoated PLA and PDA plus COL-coated scaffolds by day 21, cells seeded onto PDA plus COL scaffolds produced...Continue Reading

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Citations

Apr 6, 2020·Biotechnology and Applied Biochemistry·Hessam RezaeiMohammadali Sahebalzamani
May 20, 2020·Advanced Healthcare Materials·Fan Zhang, Martin W King
Aug 7, 2020·Nanomaterials·Chiara CavalliniLuca Menichetti
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Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Raúl Vallejos BaierJuan F Vivanco
Jun 26, 2021·Journal of Biomedical Nanotechnology·Wei JiangShuyan Zhao

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