Evaluation of bone regeneration by DNA release from composites of oligo(poly(ethylene glycol) fumarate) and cationized gelatin microspheres in a critical-sized calvarial defect

Journal of Biomedical Materials Research. Part a
F Kurtis KasperAntonios G Mikos

Abstract

This research examines the bone formation response to release of plasmid DNA encoding human Bone Morphogenetic Protein-2 from hydrogel composites consisting of cationized gelatin microspheres (CGMS) embedded within a crosslinked oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel network in a critical-sized rat cranial defect model after 30 days. Four composite groups were investigated: (1) composites with 10 microg DNA loaded into the CGMS phase, (2) composites with 10 microg DNA loaded into the OPF phase, (3) composites with 100 microg DNA loaded into the OPF phase, and (4) composites without DNA (material control). Light microscopy revealed no enhancement in bone formation for groups releasing plasmid DNA, relative to the material control group. Limited formation of new bone was observed from the defect margins and within the defect for some samples. The hydrogels swelled appreciably and fragmentation of the implants was noted to varying degrees among samples within groups, with a presence of inflammatory cells related to the degree of fragmentation. The lack of enhancement in bone formation indicates that the release of plasmid DNA from the composites was not sufficient to elicit a bone regeneration response.

References

Oct 10, 1997·Human Gene Therapy·K A Choate, P A Khavari
Jun 29, 1999·Nature Biotechnology·L D SheaD J Mooney
Sep 9, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·E WalterH P Merkle
Jan 14, 2000·Nature Biotechnology·K FuR Langer
Jan 14, 2000·Nature Biotechnology·D Luo, W M Saltzman
Jun 6, 2000·Advanced Drug Delivery Reviews·Y Ikada, Y Tabata
Jun 6, 2000·Advanced Drug Delivery Reviews·C W Pouton, L W Seymour
Sep 5, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·E WalterH P Merkle
Jan 5, 2002·Journal of Biomedical Materials Research·John P FisherJohn A Jansen
Apr 12, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yasunori FukunakaYasuhiko Tabata
Apr 12, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Teruyoshi AoyamaYasuhiko Tabata
Jun 22, 2002·Journal of Drug Targeting·Hossein HosseinkhaniYasuhiko Tabata
Jun 18, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Toshihiro KushibikiYasuhiko Tabata
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Johnna S TemenoffAntonios G Mikos
May 25, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·F Kurtis KasperAntonios G Mikos
Sep 6, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·F Kurtis KasperYasuhiko Tabata

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Citations

Jun 2, 2012·Nature Protocols·Lucas A KinardAntonios G Mikos
Sep 29, 2012·Nature Protocols·Patrick P SpicerAntonios G Mikos
Oct 23, 2014·Therapeutic Delivery·Jiankang Song, Sander Cg Leeuwenburgh
May 18, 2007·Advanced Drug Delivery Reviews·James D KretlowAntonios G Mikos
Jul 31, 2013·Journal of Biomedical Materials Research. Part a·Lucas A KinardAntonios G Mikos
Dec 9, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tiffany N VoAntonios G Mikos
Nov 26, 2016·Advanced Healthcare Materials·Sue Anne Chew, Serena Danti
Apr 25, 2018·Macromolecular Bioscience·Xiaoyuan LiYubin Huang
Feb 17, 2018·Journal of Translational Medicine·Pan WuChunmao Han
Apr 5, 2016·Tissue Engineering and Regenerative Medicine·Young-Dong KimChong-Su Cho
Feb 27, 2014·Biotechnology & Genetic Engineering Reviews·Fiona WegmanJacqueline Alblas
Jul 25, 2012·Clinical Orthopaedics and Related Research·James H HuiAntonios G Mikos
Jul 11, 2020·Biomedical Materials·Satheesh ElangovanAliasger K Salem
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin GantenbeinDevina Purmessur
Aug 27, 2021·Macromolecular Rapid Communications·Xingchen LiQuan Lin

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