Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Jia GuanShao-Ping Li

Abstract

Free and polymeric carbohydrates in Cordyceps, a valued edible mushroom and well-known traditional Chinese medicine, were determined using stepwise pressurized liquid extraction (PLE) extraction and GC-MS. Based on the optimized PLE conditions, acid hydrolysis and derivatization, ten monosaccharides, namely rhamnose, ribose, arabinose, xylose, mannose, glucose, galactose, mannitol, fructose and sorbose in 13 samples of natural and cultured Cordyceps were qualitatively and quantitatively analyzed and compared with myo-inositol hexaacetate as internal standard. The results showed that natural C. sinensis contained more than 7.99% free mannitol and a small amount of glucose, while its polysaccharides were usually composed of mannose, glucose and galactose with a molar ratio of 1.00:16.61-3.82:1.60-1.28. However, mannitol in cultured C. sinensis and cultured C. militaris were less than 5.83%, and free glucose was only detected in a few samples, while their polysaccharides were mainly composed of mannose, glucose and galactose with molar ratios of 1.00:3.01-1.09:3.30-1.05 and 1.00:2.86-1.28:1.07-0.78, respectively. Natural and cultured Cordyceps could be discriminated by hierarchical clustering analysis based on its free carbohydrat...Continue Reading

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Citations

Dec 15, 2010·Analytical and Bioanalytical Chemistry·Jia GuanShao-Ping Li
Sep 18, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ke LiXuemei Qin
May 21, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lan-Zhen MengShao-Ping Li
Jan 20, 2016·International Journal of Biological Macromolecules·Xiaocheng LvLeshuai W Zhang
May 22, 2013·Journal of Pharmaceutical and Biomedical Analysis·J ZhaoS P Li
May 23, 2015·Drug Discoveries & Therapeutics·Guangping LvShaoping Li
Dec 12, 2012·Journal of Separation Science·De-jun HuShao-ping Li
Aug 19, 2015·Journal of Pharmaceutical and Biomedical Analysis·Jianshuang ZhangXin Liu
Apr 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying DongJinglin Ding
May 12, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yi LiuChunchao Han
Nov 23, 2016·Scientific Reports·Ding-Tao WuJing Zhao
Dec 7, 2018·Journal of Asian Natural Products Research·Qing-Ying LuoZheng-You He

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