Evaluation of cell death and proliferation in psoriatic epidermis

Journal of Dermatological Science
Keisei KawashimaYoshinori Otsuki

Abstract

Previous studies of psoriatic epidermis using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) method, a type of apoptotic detection method, showed that TUNEL-positive keratinocytes were abundantly distributed in all layers of the psoriatic epidermis, although psoriasis is a hyperproliferative disorder. We sought to clarify the nature of cell kinetics in a psoriatic epidermis on the basis of differences in the reactivities in TUNEL and formamide-induced DNA denaturation assay combined with the detection of denatured DNA with a monoclonal antibody (MAb) against single-stranded DNA (formamide-MAb assay) between the normal and psoriatic epidermides. The kinetics of keratinocytes was evaluated by the immunohistochemistry of Ki-67 for proliferation activity and by TUNEL, TUNEL combined with transmission electron microscopy (TUNEL/TEM), and formamide-MAb assay for apoptosis. The number of Ki-67-positive cells in the psoriatic epidermis was significantly higher than that in the normal epidermis. In the normal epidermis, both TUNEL and formamide-MAb assay showed a similar distribution pattern, that is, both TUNEL and formamide-MAb assay-positive keratinocytes were present only in the upper ...Continue Reading

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Citations

Jul 16, 2008·Archives of Histology and Cytology·Katsura UedaSatoshi Wakisaka
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