Evaluation of cell-laden polyelectrolyte hydrogels incorporating poly(L-Lysine) for applications in cartilage tissue engineering

Biomaterials
Johnny LamA G Mikos

Abstract

To address the lack of reliable long-term solutions for cartilage injuries, strategies in tissue engineering are beginning to leverage developmental processes to spur tissue regeneration. This study focuses on the use of poly(L-lysine) (PLL), previously shown to up-regulate mesenchymal condensation during developmental skeletogenesis in vitro, as an early chondrogenic stimulant of mesenchymal stem cells (MSCs). We characterized the effect of PLL incorporation on the swelling and degradation of oligo(poly(ethylene) glycol) fumarate) (OPF)-based hydrogels as functions of PLL molecular weight and dosage. Furthermore, we investigated the effect of PLL incorporation on the chondrogenic gene expression of hydrogel-encapsulated MSCs. The incorporation of PLL resulted in early enhancements of type II collagen and aggrecan gene expression and type II/type I collagen expression ratios when compared to blank controls. The presentation of PLL to MSCs encapsulated in OPF hydrogels also enhanced N-cadherin gene expression under certain culture conditions, suggesting that PLL may induce the expression of condensation markers in synthetic hydrogel systems. In summary, PLL can function as an inductive factor that primes the cellular microenviro...Continue Reading

References

Oct 1, 1977·Developmental Biology·P B AhrensR S Reiter
Aug 1, 1975·FEBS Letters·B ObrinkB Carlsson
Mar 1, 1991·Developmental Biology·C N Coelho, R A Kosher
Jun 1, 1986·Developmental Biology·J D San Antonio, R S Tuan
Dec 1, 1994·Cell Adhesion and Communication·S A Oberlender, R S Tuan
Dec 1, 1994·Experimental Cell Research·S TavellaP Castagnola
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·I MartinL E Freed
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Nov 28, 2000·Journal of Biomedical Materials Research·K G WieseJ H Peters
Nov 10, 2001·Annual Review of Biomedical Engineering·N A PeppasJ Zhang
Dec 18, 2001·Journal of Biomedical Materials Research·Stephanie J Bryant, Kristi S Anseth
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 21, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anthony M Delise, Rocky S Tuan
Aug 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Theresa A HollandAntonios G Mikos
Mar 30, 2004·Biomaterials·Jamie OstrohaN Dan
Nov 1, 2005·The Journal of Biological Chemistry·Nobuhiro KamiyaKoji Kimata
Sep 12, 2006·Biomaterials·Daniela EyrichTorsten Blunk
Aug 11, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Shyni VargheseJennifer Elisseeff
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 1, 2010·Advanced Materials·Brandon V SlaughterNicholas A Peppas
Mar 2, 2012·Journal of Cellular Physiology·Nalinkanth V Ghone, Warren L Grayson
Jun 2, 2012·Nature Protocols·Lucas A KinardAntonios G Mikos
Nov 1, 2009·Sports Health·Alice J Sophia FoxScott A Rodeo
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Celeste ScottiIvan Martin
Apr 2, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kyobum KimF Kurtis Kasper
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Liming BianJason A Burdick
Nov 14, 2013·The American Journal of Sports Medicine·John A AndersonFarshid Guilak

❮ Previous
Next ❯

Citations

Nov 23, 2019·Journal of Biomedical Materials Research. Part a·Jason L GuoAntonios G Mikos
Mar 28, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xiaoxiao WenLei Yang
Nov 5, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Whitney C Blocher, Sarah L Perry
Jan 10, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhongliang JiangJohn Oakey
May 9, 2019·Journal of Tissue Engineering and Regenerative Medicine·Hang LinRocky S Tuan
Nov 20, 2020·International Journal of Biological Macromolecules·Penghui WangBo Chi
Feb 2, 2021·Journal of Orthopaedic Translation·Sida LiaoShibi Lu
Oct 4, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu Seon KimAntonios G Mikos
May 1, 2021·Acta Biomaterialia·Jason L GuoAntonios G Mikos
Jan 20, 2021·ACS Biomaterials Science & Engineering·Yonghui WangXingdong Zhang
Mar 21, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Nicolas HanauerXavier Banquy
Jun 24, 2020·Journal of Agricultural and Food Chemistry·Kaiquan LiuTengfei Wang
Feb 8, 2020·Journal of the American Society for Mass Spectrometry·Mehmet AtakayChrys Wesdemiotis
Nov 19, 2021·Expert Opinion on Biological Therapy·Parisa GhandforoushanGorka Orive

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.