Evaluation of cell-surface displayed synthetic consensus dengue EDIII cells as a potent oral vaccine candidate

Microbial Cell Factories
Jyotiranjan BalDae-Hyuk Kim

Abstract

Dengue is a rapidly spreading mosquito borne tropical viral disease affecting hundreds of millions of people across the globe annually. The dengue virus (DENV) includes four genetically distinct serotypes that cause serious life-threatening infections, including dengue hemorrhagic fever/dengue shock syndrome. Dengue vaccine development is complicated by the possibility of vaccine-enhanced severe dengue disease due to antibody-dependent enhancement by pre-existing cross-reactivity, as well as homotypic antibodies. Thus, the development of an efficacious dengue vaccine conferring simultaneous and durable immunity to each of the four DENV serotypes has not yet been developed despite years of research. For mass immunization in deeply affected resource-limited countries, oral vaccination is considered more beneficial than conventional approaches. Therefore, in a continuing effort towards designing economical and potent vaccine candidates, the current study applied yeast surface display technology to develop an oral dengue vaccine candidate using whole recombinant yeast cells displaying the recombinant fusion protein of M cell targeting ligand Co1 fused to the synthetic consensus dengue envelope domain III (scEDIII). Female Balb/c mi...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Bender, J R Pringle
Mar 7, 2001·Applied and Environmental Microbiology·Y M ShinM S Yang
Dec 24, 2003·Advances in Virus Research·Scott B Halstead
Dec 31, 2003·Advances in Virus Research·Brett D Lindenbach, Charles M Rice
Jan 23, 2004·Nature·Yorgo ModisStephen C Harrison
Dec 23, 2004·Journal of Virology·Yorgo ModisStephen C Harrison
Jun 15, 2007·Nature Reviews. Microbiology·Stephen S WhiteheadBrian R Murphy
Jun 6, 2008·Expert Opinion on Drug Delivery·Rhonda Kuolee, Wangxue Chen
Jul 5, 2008·Journal of Immunotherapy·Shanshan W HowlandKarl Dane Wittrup
Jul 31, 2008·Expert Review of Vaccines·Lina Wang, Ross L Coppel
Dec 31, 2008·Microbes and Infection·Chih-Hsiang LengHsin-Wei Chen
Jan 30, 2010·Expert Review of Vaccines·Maria G GuzmanGerardo Guillén
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sae-Hae KimYong-Suk Jang
Sep 3, 2011·European Journal of Immunology·Sae-Hae KimYong-Suk Jang
Nov 17, 2011·Vaccine·Vincent PavotStéphane Paul
Jun 2, 2012·Applied Microbiology and Biotechnology·Tsutomu TanakaAkihiko Kondo
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Jul 23, 2013·Pathogens and Disease·Seiji ShibasakiMitsuyoshi Ueda
Aug 30, 2013·Expert Review of Vaccines·Monica A McArthurRobert Edelman
Jan 1, 2014·Advances in Virus Research·Lauren E Yauch, Sujan Shresta
Feb 21, 2014·Human Vaccines & Immunotherapeutics·Rebecca De SmetClaude A Cuvelier
Mar 15, 2014·Experimental & Molecular Medicine·Sae-Hae Kim, Yong-Suk Jang
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Miao WangYongguang Zhang
May 8, 2015·PLoS Neglected Tropical Diseases·Nicole L AcheeThomas W Scott
Jun 11, 2015·Methods in Molecular Biology·Gerald M Cherf, Jennifer R Cochran
Jul 28, 2015·The New England Journal of Medicine·Sri Rezeki HadinegoroUNKNOWN CYD-TDV Dengue Vaccine Working Group
Sep 19, 2015·Science·Leah C KatzelnickDerek J Smith
Jan 13, 2017·Journal of Immunology Research·Han LeiKaiming Ye
Feb 18, 2018·Microbial Cell Factories·Jyotiranjan BalDae-Hyuk Kim

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Methods Mentioned

BETA
PCR
Protein Assay
electrophoresis
enzyme-linked immunosorbent assay
ELISAs
ELISA

Software Mentioned

GraphPad Prism

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Antibody-Dependent Enhancement

Antibody-dependent enhancement of viral infection is the entry of virus into host cells mediated by antiviral antibodies interacting with Fc or complement receptors. This has been most extensively observed with the dengue virus. Find the latest research on antibody-dependent enhancement here.