Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
A P C RibeiroA J L Pombeiro

Abstract

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

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Citations

Sep 12, 2018·Nanomaterials·Alexandra-Cristina BurdușelEcaterina Andronescu
Jul 18, 2018·Frontiers in Microbiology·Pedro V BaptistaAlexandra R Fernandes
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea RónaváriMonika Kiricsi
Nov 25, 2020·Journal of Medicinal Chemistry·Mariana MoraisJoão A V Prior
Dec 5, 2020·The Journal of Physical Chemistry Letters·Pradeep KaduSamir K Maji

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