Evaluation of cellular adhesion and organization in different microporous polymeric scaffolds

Biotechnology Progress
Amish AsthanaWilliam S Kisaalita

Abstract

The lack of prediction accuracy during drug development and screening risks complications during human trials, such as drug-induced liver injury (DILI), and has led to a demand for robust, human cell-based, in vitro assays for drug discovery. Microporous polymer-based scaffolds offer an alternative to the gold standard flat tissue culture plastic (2D TCPS) and other 3D cell culture platforms as the porous material entraps cells, making it advantageous for automated liquid handlers and high-throughput screening (HTS). In this study, we optimized the surface treatment, pore size, and choice of scaffold material with respect to cellular adhesion, tissue organization, and expression of complex physiologically relevant (CPR) outcomes such as the presence of bile canaliculi-like structures. Poly-l-lysine and fibronectin (FN) coatings have been shown to encourage cell attachment to the underlying substrate. Treatment of the scaffold surface with NaOH followed with a coating of FN improved cell attachment and penetration into pores. Of the two pore sizes we investigated (A: 104 ± 4 μm; B: 175 ± 6 μm), the larger pore size better promoted cell penetration while limiting tissue growth from reaching the hypoxia threshold. Finally, polysty...Continue Reading

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Citations

Sep 12, 2018·Cell Division·Cecil J GomesJesse D Martinez
Aug 20, 2019·ACS Applied Polymer Materials·Matilde PuttiPatricia Y W Dankers
Jan 20, 2021·ACS Biomaterials Science & Engineering·Amish AsthanaWilliam S Kisaalita

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