Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro

Parasitology Research
Chen-Chieh LiaoJyh-Wei Shin

Abstract

Blastocystis is a parasitic protist with a worldwide distribution that is commonly found in patients with colon and gastrointestinal pathological symptoms. Blastocystis infection has also commonly been reported in colorectal cancer and HIV/AIDS patients with gastrointestinal symptoms. To understand the pathway networks of gene regulation and the probable mechanisms influencing functions of HT-29 host cells in response to parasite infection, we examined the expression of 163 human oncogenes and kinases in human colon adenocarcinoma HT-29 cells co-incubated with Blastocystis by in-house cDNA microarray and PCR analysis. At least 10 genes were shown to be modified following Blastocystis co-incubation, including those with immunological, tumorigenesis, and antitumorigenesis functions. The expression of genes encoding cellular retinoic acid binding protein 2 (CRABP2) and proliferating cell nuclear antigen (PCNA) was markedly upregulated and downregulated, respectively. Reverse transcriptase-PCR validated the modified transcript expression of CRABP2 and other associated genes such as retinoic acid (RA)-related nuclear-receptor (RARα). Together, our data indicate that CRABP2, RARα, and PCNA expressions are involved in RA signaling reg...Continue Reading

References

Sep 1, 1991·Journal of Clinical Microbiology·M J Lee
May 1, 1991·Infection·P L GaravelliM Libanore
Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Apr 1, 1987·Clinical and Experimental Pharmacology & Physiology·S Y Guirges, N S Al-Waili
Jan 1, 1997·Parasitology Research·K T MoeE H Yap
Oct 12, 2005·Biotechnology Annual Review·Michael V BerridgeAn S Tan
Jan 31, 2006·Cancer Letters·Peter J Coopman, Susette C Mueller
Feb 10, 2006·The New England Journal of Medicine·Israel F Charo, Richard M Ransohoff
Aug 19, 2006·BMC Bioinformatics·Srinivas Veerla, Mattias Höglund
Feb 6, 2008·Environmental Toxicology·Sung-Hwa SohnMeyoung-Kon Kim
Feb 19, 2010·Parasitology Research·Samudi ChandramathiUmah Rani Kuppusamy
Jun 3, 2011·The American Journal of Tropical Medicine and Hygiene·Christen Rune StensvoldHenrik Vedel Nielsen

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