Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Viruses
Boris PastorinoRemi N Charrel

Abstract

Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.

References

May 26, 2004·Journal of Virological Methods·Jamie A BlowChristopher N Mores
Aug 23, 2011·Applied and Environmental Microbiology·Jose-Luis SagripantiHans-Jürgen Marschall
Jul 17, 2015·Journal of Clinical Microbiology·Sophie J SmitherMark S Lever
Apr 14, 2016·Emerging Infectious Diseases·Elaine HaddockHeinz Feldmann
Feb 9, 2020·Journal of Virological Methods·Britta A WoodMichael Eschbaumer
Feb 20, 2020·The New England Journal of Medicine·Lirong ZouJie Wu
Feb 28, 2020·The Lancet Infectious Diseases·Yang PanQuanyi Wang
Mar 18, 2020·The New England Journal of Medicine·Neeltje van DoremalenVincent J Munster
Apr 29, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Bernard La ScolaDidier Raoult
May 23, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jared BullardGuillaume Poliquin
Jun 21, 2020·Biosafety and Health· National Health Commission Of The People's Republic Of China, UNKNOWN Bureau of Disease Prevention and Control, National Health Commission of People's Republic of Chinabiosafetyandhealth@ivdc.ch

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Citations

Aug 28, 2020·Journal of Crohn's & Colitis·Shadi NayeriMark S Silverberg
Dec 9, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Avrill M AsplandSherry Thornton
Jan 14, 2021·Revue Francophone Des Laboratoires : RFL·Paul Hofman
Jan 13, 2021·Methods and Protocols·Kaitlyn A BarrowJason S Debley
Feb 1, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad AbbasJeffrey A Stuart
Mar 7, 2021·Methods and Protocols·Ranjeet KumarSelvakumar Subbian
Apr 1, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Alex F CarvalhoFlavio G da Fonseca
May 27, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Avrill AsplandMichael Solga
Jul 19, 2021·Diagnostic Microbiology and Infectious Disease·Shairaz BakshMao-Cheng Lee

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Methods Mentioned

BETA
nucleic acid extraction

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