Evaluation of chemically induced toxicity using an in vitro model of human corneal epithelium

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S L WardS D Dimitrijevich

Abstract

Stratified cultures of human corneal epithelial cells were used as an in vitro model for the evaluation of chemical damage to the ocular surface. Plasmid-transfected human corneal epithelial cells (HCE-T cells; 10.014 pRSV-T), cultured on a collagen membrane at the air-liquid interface, form a stratified epithelium (the HCE-T model). Results showed the HCE-T cell line to be comparable to primary human corneal epithelial (HCE) cells in morphology, keratin expression, and calcium-mediated modulation of morphology. Intercellular junctions and other ultrastructural features common to human corneal epithelium were identified in stratified HCE-T cultures. Chemical effects on morphology and cell viability indicated that the HCE-T model was more resistant to chemical toxicity than HCE-T monolayer cultures. Barrier function established by the HCE-T model was determined by measuring transepithelial permeability to sodium fluorescein (TEP) and transepithelial electrical resistance (TER). Previous results demonstrated similar baseline TEP and TER values for HCE and HCE-T cultures. Stratified HCE-T cultures retained 96.4 +/- 2.2% of the fluorescein applied to the apical surface for 30 min, and attained a TER of 468 +/- 89 ohms x cm(2); thes...Continue Reading

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Citations

Aug 3, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Manlong XuDavid J McCanna
Aug 8, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Ana Guzman-AranguezJesús Pintor
Feb 3, 2009·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Philip B Morgan, Carole Maldonado-Codina
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May 29, 2016·Biochimica Et Biophysica Acta·Yan LiFengshan Wang
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Jan 9, 2003·Current Eye Research·Kumar SinniahJohn Ubels
Jul 12, 2005·European Journal of Cell Biology·Pauline MooreRichard H Clothier
Aug 17, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jennifer L DumouchelUpendra A Argikar
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Oct 31, 2002·Alternatives to Laboratory Animals : ATLA·Richard ClothierLinda Noble

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