Evaluation of Chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks.

PLoS Neglected Tropical Diseases
Grace YapLee-Ching Ng

Abstract

The sensitivity and specificity of two in-house MAC-ELISA assays were tested and compared with the performance of commercially-available CTK lateral flow rapid test and EUROIMMUN IFA assays for the detection of anti-Chikungunya virus (CHIKV) IgM. Each MAC-ELISA assay used a whole virus-based antigen derived from genetically distinct CHIKV strains involved in two chikungunya disease outbreaks in Singapore (2008); a January outbreak strain with alanine at amino acid residue 226 of the E1 glycoprotein (CHIKV-A226) and a May-to-September outbreak strain that possessed valine at the same residue (CHIKV-226V). We report differences in IgM detection efficacy of different assays between the two outbreaks. The sensitivities of two PCR protocols were also tested. For sera from January outbreak, the average detection threshold of CTK lateral flow test, MAC-ELISAs and EUROIMMUN IFA assays was 3.75, 4.38 and 4.88 days post fever onset respectively. In contrast, IgM detection using CTK lateral flow test was delayed to more than 7 days after fever onset in the second outbreak sera. However, MAC-ELISA using CHIKV-226V detected IgM in the second outbreak sera 3.96 days after fever onset, which was approximately one day earlier compared to the s...Continue Reading

References

May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Mar 26, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M PfefferR M Kinney
May 12, 2006·Indian Journal of Medical Microbiology·V Ravi
Jun 27, 2006·Vector Borne and Zoonotic Diseases·Stephen Higgs
Dec 13, 2006·BMJ : British Medical Journal·Suranjith L Seneviratne, Jennifer Perera
Jun 8, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S R SanthoshP V Lakshmana Rao
Sep 20, 2007·BMJ : British Medical Journal·Jo Lines
Dec 12, 2007·PLoS Pathogens·Konstantin A TsetsarkinStephen Higgs
Jan 22, 2008·Journal of Medical Virology·Christophe N PeyrefitteMarc Grandadam
Feb 26, 2008·Journal of Virological Methods·Nadine LitzbaMatthias Niedrig
May 15, 2008·Viral Immunology·John T RoehrigAlan D T Barrett
Feb 26, 2009·Emerging Infectious Diseases·Marcus PanningChristian Drosten
May 1, 2009·Emerging Infectious Diseases·Yee S LeoLee C Ng
Sep 16, 2009·Emerging Infectious Diseases·Lee-Ching NgSeow-Poh Khoo
Dec 4, 2009·The Journal of General Virology·H C HapuarachchiLee-Ching Ng
May 29, 2010·Annals of Tropical Medicine and Parasitology·V AuksornkittiN Luplertlop
Aug 2, 2011·Asian Pacific Journal of Tropical Medicine·Kamol SuwannakarnYong Poovorawan

❮ Previous
Next ❯

Citations

Jul 26, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A Requena-MéndezJ Gascón
Nov 25, 2014·Emerging Infectious Diseases·Christine M PratIsabelle Leparc-Goffart
May 17, 2011·PLoS Neglected Tropical Diseases·Grace YapLee-Ching Ng
Nov 10, 2013·PLoS Neglected Tropical Diseases·Herman KosasihMaya Williams
Feb 2, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sonja JacobsenMatthias Niedrig
Jan 3, 2013·Journal of Travel Medicine·Chantal B E M ReuskenMarion G P Koopmans
Jun 23, 2015·Best Practice & Research. Clinical Rheumatology·Ashish Jacob Mathew, Vinod Ravindran
Nov 22, 2011·Lancet·Felicity J BurtMark T Heise
Apr 22, 2015·Vector Borne and Zoonotic Diseases·Fabio MaguranoAlessandro Bartoloni
Apr 22, 2015·Vector Borne and Zoonotic Diseases·I-Ching SamSazaly AbuBakar
Jan 28, 2015·Emerging Infectious Diseases·Masri Sembiring MahaUNKNOWN Subangkit
Jun 28, 2016·Emerging Infectious Diseases·Elisa BurdinoValeria Ghisetti
Dec 3, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Virginie RougeronPierre Roques
Jan 30, 2015·Revista Da Sociedade Brasileira De Medicina Tropical·Mario Luis Garcia de Figueiredo, Luiz Tadeu Moraes Figueiredo
Feb 5, 2014·The American Journal of Tropical Medicine and Hygiene·Sarunyou ChusriRichard G Jarman
Mar 16, 2016·The American Journal of Tropical Medicine and Hygiene·Barbara W JohnsonMichael A Drebot
Mar 28, 2018·The American Journal of Tropical Medicine and Hygiene·Matthew L RobinsonVidya Mave
Apr 7, 2015·Infection & chemotherapy·Jeong-Hwan Hwang, Chang-Seop Lee
Apr 19, 2019·Journal of Clinical Microbiology·Muktha S NatrajanJesse J Waggoner
May 26, 2011·Expert Review of Anti-infective Therapy·Alexander C OuthredDominic E Dwyer
Feb 23, 2020·Applied Microbiology and Biotechnology·Rajesh KumarHilal Ahmad Parray
May 9, 2012·Nature Reviews. Rheumatology·Andreas SuhrbierPhilippe Gasque
Nov 22, 2017·Turkish Journal of Medical Sciences·Tuğba AtalayAhmet Kürşat Azkur
Dec 7, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew L RobinsonVidya Mave
Feb 10, 2017·PloS One·Chong-Long ChuaYoke-Fun Chan
Jul 26, 2018·Virology Journal·Marcílio Jorge FumagalliLuiz Tadeu Moraes Figueiredo
Nov 26, 2011·Expert Review of Anti-infective Therapy·I-Ching SamYoke Fun Chan
Dec 7, 2016·The Journal of Infectious Diseases·Barbara W JohnsonChristin H Goodman
May 31, 2018·PLoS Neglected Tropical Diseases·Benjamin Lopez-JimenaManfred Weidmann
Jun 2, 2020·PLoS Neglected Tropical Diseases·Mansyur ArifMuhammad Karyana
Mar 27, 2019·Viruses·Kelli L Barr, Vedana Vaidhyanathan
Jun 27, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Line Lobaloba IngobaFrancine Ntoumi

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR
ELISA
ELISAs

Software Mentioned

JEMBOSS ( ANTIGENIC )
Excel
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Lancet Infectious Diseases
Gilles PialouxMichel Strobel
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Gianandrea BorgheriniFabrice Paganin
© 2021 Meta ULC. All rights reserved