Evaluation of chromogenic factor IX assays by automated protocols

Haemophilia : the Official Journal of the World Federation of Hemophilia
G KershawT-L Khoo

Abstract

Chromogenic substrate assays (CSA) to measure Factor IX (FIX) have recently become commercially available. However, information on their performance characteristics and use in diagnostic haemostasis laboratories remains limited. To evaluate the Hyphen Biomed (Hyphen) and Rossix FIX CSAs on fully automated coagulation analysers and compare them to the FIX one-stage assay (OSA). This study was conducted in a tertiary referral haemostasis laboratory associated with a haemophilia treatment centre. Automated CSA protocols were adapted to the Sysmex CS2500 (CS2500) and Diagnostica Stago STA-R (STA-R) analysers. Samples assayed were from healthy volunteers, haemophilia B patients and FIX deficient plasma spiked with either plasma derived, recombinant or extended half-life FIX products. Reference intervals for Hyphen and Rossix assays were 73 IU/dL to 164 IU/dL and 73 IU/dL to 168 IU/dL, respectively, on the CS2500 analyser; and 84 IU/dL to 165 IU/dL for the Rossix assay on the STA-R. Repeatability across all method/analyser combinations resulted in CVs ranging from 0.8% to 5.4%. Between run reproducibility gave CVs <6.7% for all method/analyser combinations. In spiked samples, FIX recoveries were mostly within an acceptable limit of 1...Continue Reading

References

Jun 28, 2003·Haemophilia : the Official Journal of the World Federation of Hemophilia·T W Barrowcliffe
Apr 26, 2006·Journal of Thrombosis and Haemostasis : JTH·S CinottiM Morfini
Sep 25, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·C PouplardY Gruel
Jun 30, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·T W Barrowcliffe
Sep 18, 2012·International Journal of Laboratory Hematology·I MackieUNKNOWN British Committee for Standards in Haematology
Apr 26, 2014·Haemophilia : the Official Journal of the World Federation of Hemophilia·S KitchenK Mertens
May 8, 2014·Haemophilia : the Official Journal of the World Federation of Hemophilia·H V WilmotE Gray
Feb 18, 2015·Haemophilia : the Official Journal of the World Federation of Hemophilia·S KitchenN S Key
Jan 26, 2016·Seminars in Hematology·Rajiv K Pruthi
Jul 14, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·S KitchenS Tiefenbacher
Mar 7, 2017·Seminars in Thrombosis and Hemostasis·Steve KitchenRobert Gosselin
Apr 26, 2017·Haemophilia : the Official Journal of the World Federation of Hemophilia·K KihlbergJ Astermark

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Citations

Jul 5, 2019·International Journal of Laboratory Hematology·Atsuo SuzukiTadashi Matsushita
Mar 7, 2020·Haemophilia : the Official Journal of the World Federation of Hemophilia·Stella C Williams, Elaine Gray
Mar 24, 2020·International Journal of Laboratory Hematology·Jurg M SommerAnnemieke J Willemze
Apr 22, 2020·Haemophilia : the Official Journal of the World Federation of Hemophilia·Thomas SinegreAurélien Lebreton
Apr 12, 2020·European Journal of Haematology·Emmanuelle JeanpierreUNKNOWN French Study Group on the Biology of Hemorrhagic Diseases (the BIMHO group)
Apr 24, 2019·Research and Practice in Thrombosis and Haemostasis·Carmen Escuriola EttingshausenRobert Klamroth
Apr 30, 2019·International Journal of Laboratory Hematology·Sema GencBeyhan Ömer
Nov 30, 2020·European Journal of Pharmacology·Senade AtsouGeorges Jourdi

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