Evaluation of chromosomal aberrations in patients with benign conditions and reactive changes in urinary cytology

Cancer Cytopathology
Coya TapiaLukas Bubendorf

Abstract

Fluorescence in situ hybridization (FISH) is routinely used to help clarify atypical urinary cytology. However, to the authors' knowledge, little is known regarding the frequency of chromosomal aberrations in non-neoplastic conditions that could potentially lead to false-positive FISH results. The objective of the current study was to evaluate the frequency of chromosomal aberrations in benign cells of the urinary tract using the UroVysion FISH test. The authors analyzed 77 Papanicolaou-stained benign urine cytology specimens with reactive epithelial atypia using a FISH assay detecting the chromosomes 3, 7, and 17 and the gene locus 9p21. A positive test result was defined as an increased copy number of at least 2 chromosomes in ≥ 4 of 25 cells, or > 10 cells with a tetraploid or octaploid pattern, or homozygous or heterozygous deletion of 9p21 (≥ 12cells). FISH was positive in 27 of 77 bladder washings (35.1%) including 25 of 65 bladder washings (40.5%) and 2 of 15 voided urines (13.5%) from patients with irritative bladder (15 of 36 patients), a history of radiotherapy (7 of 12 patients), nonspecific cystitis (3 of 11 patients), hematuria (3 of 8 patients), and lithiasis (1 of 4 patients) . In 7 of 27 FISH-positive urothelial...Continue Reading

References

Nov 1, 1989·International Journal of Radiation Oncology, Biology, Physics·A B JacobsenS D Fosså
Feb 1, 1973·Journal of the Neurological Sciences·D M Mann, P O Yates
Dec 1, 1994·The Journal of Urology·W BushmanA J Schaeffer
Jan 1, 1996·International Urology and Nephrology·S BaltaciO Göğüş
Jan 26, 1999·International Journal of Oncology·K JunkerU Claussen
Apr 3, 2001·Nature Reviews. Genetics·T Cremer, C Cremer
Jul 13, 2001·American Journal of Clinical Pathology·Lukas BubendorfPeter Dalquen
Mar 11, 2003·The Journal of Biological Chemistry·Jacques-Emmanuel GuidottiChantal Desdouets
Jul 2, 2003·Nature Genetics·Thoru Pederson
Feb 24, 2005·Cancer Biology & Therapy·Valentina GuasconiSlimane Ait-Si-Ali
Jun 11, 2005·The Journal of Urology·Kristin ChrouserMichael Blute
Jun 22, 2005·Acta Cytologica·Leopold G Koss
Dec 7, 2005·Journal of Cellular Physiology·Hao G Nguyen, Katya Ravid
Apr 29, 2006·International Journal of Cancer. Journal International Du Cancer·Tobias ZellwegerLukas Bubendorf
Jul 7, 2007·Thyroid : Official Journal of the American Thyroid Association·Shin-Ichi MurataRyohei Katoh
Jul 21, 2007·Human Pathology·Kevin C Halling, Benjamin R Kipp
Dec 29, 2007·European Urology·Olaf P J Vrooman, J Alfred Witjes
Aug 30, 2008·Advances in Anatomic Pathology·Kevin C Halling, Benjamin R Kipp
Nov 4, 2009·Genes & Development·Hyun O LeeRobert J Duronio
Feb 10, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Stefan LönnRochelle E Curtis
May 18, 2010·European Urology·Christine MianArmin Pycha

Citations

Sep 13, 2013·Der Urologe. Ausg. A·M BöhmArbeitskreis Urinzytologie der DGU (Deutsche Gesellschaft für Urologie)
Dec 19, 2012·Annales de pathologie·Lukas Bubendorf, Eric Piaton
Sep 6, 2013·Diagnostic Cytopathology·Deloar HossainDavid G Bostwick
May 28, 2016·Archives of Pathology & Laboratory Medicine·Amy G ZhouLloyd M Hutchinson
Aug 2, 2016·Archives of Pathology & Laboratory Medicine·Spasenija Savic, Lukas Bubendorf
May 28, 2016·Advances in Anatomic Pathology·Güliz A BarkanDorothy L Rosenthal
Aug 10, 2017·Der Pathologe·Spasenija SavicLukas Bubendorf

Related Concepts

Aneuploid Cell
Urinary Bladder
Malignant Neoplasm of Urinary Bladder
Autosome Abnormalities
Chromosomes, Human
Cytodiagnosis
False Positive Reactions
Staining and Labeling
Fluorescent in Situ Hybridization
Urothelium

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.