Evaluation of chromosomal aberrations induced by 188 Re-dendrimer nanosystem on B16f1 melanoma cells

International Journal of Radiation Biology
Marcos TassanoPablo Cabral

Abstract

To study the rhenium-188 labeling of polyamidoamine (PAMAM) generation 4 (G4) dendrimer and its evaluation on biodistribution and chromosomal aberrations in melanoma cells induced by ionizing radiation as potential treatment agent. Dendrimers were first conjugated with Suc-HYNIC (succinimidyl 6-hydrazinopyridine-3-carboxylic acid hydrochloride). Dendrimer-HYNIC was then incubated with 188ReO4-. Biodistribution was performed administrating 188Re-dendrimer to normal (NM) or melanoma-bearing mice (MBM). Chromosome aberration test was conducted in order to measure treatment capacity of 188Re-dendrimer in melanoma cells. Radiolabeling yield of dendrimer was approx. 70%. Biodistribution studies in NM showed blood clearance with hepatic and renal depuration. MBM showed a similar pattern of biodistribution with tumor uptake of 6% of injected dose. Aberrant metaphases quantified in control cells were 7%, increasing to 29.5% in cells treated with 15μCi (0.555 MBq) of 188Re-dendrimer for 24 h. 188Re-dendrimer can produce double-stranded breaks in DNA induced by ionizing radiation in melanoma cells in vitro.

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Citations

Dec 13, 2019·Macromolecular Bioscience·Tingting XiaoMingwu Shen
Jan 26, 2021·Chemical Society Reviews·Juan PellicoRafael T M de Rosales
Feb 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberta CassanoSonia Trombino

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