Evaluation of chrono-impedance technique as transduction method for a carbon paste/glucose oxidase (CP/GOx) based glucose biosensor

Biosensors & Bioelectronics
Carmen C Mayorga MartinezCarmelo C Felice

Abstract

The chrono-impedance technique (CIT) for real time determination of glucose concentration in a first generation glucose oxidase/carbon paste electrode was implemented. The biosensor was polarized with a signal composed of 900 mV DC potential and 50 mV(RMS) AC signal at 0.4 Hz. A frequency response analyzer was used to measure the complex impedance (magnitude |Z| and phase (Φ)) of the biosensor-bulk interface. Real time measurements were performed while glucose was added to the bulk within a concentration range of 0-40 mM. The cumulative impedance dose-response curves were used to construct calibration curves, both for magnitude and phase. The best fitting was obtained with a hyperbolic equation. Four biosensors were built obtaining five calibration curves for each of them. A single test measurement (unknown glucose concentration) was also obtained after each calibration procedure. Glucose concentrations were estimated with the calibration curves and also measured by colorimetry, the latter being the reference method. Besides, one-way ANOVA test evaluated repeatability. Difference between means was not statistically significant (p>0.01) for both magnitudes (|Z| and Φ). The Student's t-test assessed the differences significance, ...Continue Reading

Citations

Jan 15, 2014·Biosensors & Bioelectronics·Carmen C Mayorga-MartinezArben Merkoçi
Sep 26, 2015·Angewandte Chemie·Carmen C Mayorga-MartinezMartin Pumera
Dec 21, 2011·Chemical Communications : Chem Comm·Carmen C Mayorga-MartinezArben Merkoçi
Apr 4, 2021·Sensors·Ricardo Matias TrujilloRossana Elena Madrid

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