Evaluation of cMET aberration by immunohistochemistry and fluorescence in situ hybridization (FISH) in triple negative breast cancers

Annals of Diagnostic Pathology
Mopei WangHong Zhang

Abstract

To evaluate the incidence of cMET proto-oncogene aberration in a cohort of triple negative breast cancers using immunohistochemistry and fluorescence in situ hybridization (FISH) methods and correlated with patient outcome. One hundred and six female patients with diagnosis of triple negative invasive breast carcinoma at The University of Texas-M D Anderson Cancer Center from 1983 to 2009 were included in the study. Expression of cMET was assessed by IHC using rabbit monoclonal anti-total cMET antibody (SP44 from Ventana). Staining intensity was scored on a scale of 0, 1+, 2+ and 3+. cMET overexpression was defined as at least moderate membranous/cytoplasmic staining in ≥50% of tumor cells (score ≥ 2+). FISH analysis was performed using MET (7q31) specific probe (BAC clone RP11-95i20, Abbott Molecular Inc.) and the centromere probe (CEP7/D7Z1, Abbott Molecular Inc.) as internal control. cMET amplification was defined as gene copy numbers ≥4 per cell or cMET/CEP7 ratio ≥ 2. cMET status was tested for correlation using Fisher's exact test with other clinicopathological parameters. The Kaplan-Meier product limit method was used to estimate the survival outcomes. Cox proportional hazards models were fit to determine the association...Continue Reading

Citations

Jul 10, 2019·Cancer Biomarkers : Section a of Disease Markers·Yong-Yao GuZhi-Gang Peng
Sep 13, 2019·Critical Reviews in Clinical Laboratory Sciences·Fatemeh MoosaviOmidreza Firuzi
Apr 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia Magdalena ChrzanowskaMarzena Anna Lewandowska
Oct 30, 2020·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kazuhiro SentaniWataru Yasui
Feb 5, 2021·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Wonyoung ChoiJi-Youn Han
Aug 31, 2021·Annals of Diagnostic Pathology·Huihua LiRicardo V Lloyd

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