Evaluation of combination of BACTEC mycobacteria growth indicator tube 960 system and Ogawa media for mycobacterial culture

The Korean journal of laboratory medicine
Eunsin BaeTae Sun Shim

Abstract

The combined use of liquid media and solid media is recommended for mycobacterial culture. We evaluated diagnostic performance of combination of BACTEC Mycobacteria Growth Indicator Tube (MGIT; Becton Dickinson, USA) and 2% Ogawa media (Korean Institute of Tuberculosis, Korea) for recovery of mycobacteria. In September 2007, 1,764 specimens from 1,059 patients were cultured with MGIT and Ogawa. Acid fast bacilli (AFB) smear was fluorochrome-stained. The isolates were identified into Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) with PCR using Seeplex TB Detection Kit (Seegene, Korea). Recovery rate, time to detection (TTD), contamination rate, mixed growth rate and species distribution were analyzed. Two hundred thirty-five specimens (13.3%) from 165 patients (15.6%) were positive for mycobacterial culture. Recovery rates of mycobacteria from the group using both media, MGIT only, and Ogawa only were 13.3%, 12.1%, and 7.8%, respectively. While MGIT recovered 98.9% of MTB and 79.7% of NTM, Ogawa recovered 65.9% of MTB and 54.1% of NTM. TTDs of total mycobacteria/MTB/NTM in MGIT and Ogawa were 10.6/11.4/9.7 days and 31/29/33 days, respectively. MGIT TTDs of total mycobacteria/MTB/NTM from AFB-positive spe...Continue Reading

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Citations

Nov 23, 2013·European Journal of Microbiology & Immunology·S K Parida
Feb 4, 2016·Transplant International : Official Journal of the European Society for Organ Transplantation·Jung-Wan YooTae Sun Shim
Jan 6, 2015·The Journal of Microbiology·Hye-young WangHyeyoung Lee
Jan 26, 2017·Tuberculosis and Respiratory Diseases·Yousang KoYong Bum Park
Jun 20, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hye-Young WangHyeyoung Lee
Sep 9, 2017·Journal of Clinical Laboratory Analysis·Jae-Sik JeonJong Wan Kim
Feb 6, 2018·Journal of Clinical Laboratory Analysis·Jae-Sik JeonJong Wan Kim
Oct 27, 2012·Tuberculosis and Respiratory Diseases·Jung-Wan YooTae Sun Shim
Jul 11, 2012·Annals of Laboratory Medicine·Won-Jung KohNam Yong Lee
Nov 15, 2019·Journal of Clinical Microbiology·Guohui XiaoGuoliang Zhang

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