Evaluation of conjunctival swab as a mass-screening tool for molecular diagnosis of canine visceral leishmaniasis

Parasitology Research
Rodrigo Souza LeiteAntero Silva Ribeiro de Andrade

Abstract

The canine visceral leishmaniasis (CVL) diagnosis is an important step of visceral leishmaniasis control program in Brazil once the dog is the main reservoir host of the disease. The aim of this study was to evaluate the conjunctival swab (CS) as a mass-screening tool for CVL molecular diagnosis in an endemic area classified as priority for the Brazilian Ministry of Healthy for surveillance action. A total of 1350 domiciled dogs were screened. The animals were evaluated by serological tests (enzyme-linked immunosorbent assay (ELISA) as screening and immunofluorescence antibody test (IFAT) for confirmation) and by CS associated to real-time PCR, using primers addressed to kinetoplast DNA (kDNA) minicircles and SYBR Green. Canine β-globin gene amplification was used to evaluate the sample DNA integrity. A subgroup of 484 animals was also submitted to clinical evaluation. Among the 1350 dogs screened, 369 (27.3%) were positive by CS real-time PCR and 126 (9.3%) tested positive by ELISA. Thirty-one percent (39/126) of the ELISA-positive dogs were confirmed by IFAT. CS real-time PCR was able to detect infection in dogs independently of the symptomatology degree (p > 0.05), while ELISA was more sensitive in the group of dogs that pre...Continue Reading

References

Aug 17, 1999·Journal of Clinical Microbiology·S RealeG Vesco
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·Rosângela Barbosa-De-DeusCarlos Alberto Pereira Tavares
Apr 30, 2004·The Journal of Infectious Diseases·Dalit Strauss-AyaliGad Baneth
Nov 6, 2004·Journal of Clinical Microbiology·Charles MaryHenri Dumon
Dec 8, 2006·The Veterinary Journal·Y M GomesL C Alves
Feb 5, 2008·Veterinary Parasitology·Sidney de Almeida FerreiraAntero Silva Ribeiro de Andrade
Jun 3, 2008·Trends in Parasitology·Gad BanethLluis Ferrer
Jul 8, 2008·The Veterinary Journal·Milena de Paiva CavalcantiFrederico G C Abath
Sep 16, 2008·Veterinary Parasitology·C Maia, L Campino
Mar 21, 2009·Research in Veterinary Science·Marcia Maria PilattiAntero Silva Ribeiro de Andrade
Mar 17, 2010·Veterinary Parasitology·Rodrigo Souza LeiteAntero Silva Ribeiro de Andrade
Apr 14, 2010·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Juliana de AssisWilma Aparecida Starke Buzetti
Apr 14, 2010·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Nina M G P de QueirozWilma A Starke-Buzetti
Mar 1, 2012·Revista Da Sociedade Brasileira De Medicina Tropical·José Cláudio Carneiro de FreitasLigene Fernandes de Oliveira
Apr 27, 2013·Diagnostic Microbiology and Infectious Disease·Arleana B P F AlmeidaMaria F Madeira
Feb 4, 2014·Diagnostic Microbiology and Infectious Disease·Aline Leandra Carvalho FerreiraAntero Silva Ribeiro de Andrade

❮ Previous
Next ❯

Citations

Jul 3, 2016·Veterinary Parasitology·Mariana AscharVania Lucia Ribeiro da Matta
Jun 13, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Murilo Antônio FernandesTrícia Maria Ferreira de Sousa Oliveira
Jun 20, 2019·Frontiers in Veterinary Science·Javier Moreno

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Diagnostic Microbiology and Infectious Disease
Aline Leandra Carvalho FerreiraAntero Silva Ribeiro de Andrade
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
Rodrigo Souza LeiteAntero Silva Riberiro de Andrade
© 2021 Meta ULC. All rights reserved