Evaluation of contrasting techniques for X-ray imaging of velvet worms (Onychophora)

Journal of Microscopy
Henry JahnJörg U Hammel

Abstract

Non-invasive imaging techniques like X-ray computed tomography have become very popular in zoology, as they allow for simultaneous imaging of the internal and external morphology of organisms. Nevertheless, the effect of different staining approaches required for this method on samples lacking mineralized tissues, such as soft-bodied invertebrates, remains understudied. Herein, we used synchrotron radiation-based X-ray micro-computed tomography to compare the effects of commonly used contrasting approaches on onychophorans - soft-bodied invertebrates important for studying animal evolution. Representatives of Euperipatoides rowelli were stained with osmium tetroxide (vapour or solution), ruthenium red, phosphotungstic acid, or iodine. Unstained specimens were imaged using both standard attenuation-based and differential phase-contrast setups to simulate analyses with museum material. Our comparative qualitative analyses of several tissue types demonstrate that osmium tetroxide provides the best overall tissue contrast in onychophorans, whereas the remaining staining agents rather favour the visualisation of specific tissues and/or structures. Quantitative analyses using signal-to-noise ratio measurements show that the level of ...Continue Reading

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Citations

Dec 12, 2019·Royal Society Open Science·Ivo de Sena OliveiraGeorg Mayer
Dec 16, 2018·Scientific Reports·Mark MüllerMadleen Busse
May 22, 2019·Zoological Letters·Vladimir GrossFranz Pfeiffer
Apr 5, 2019·Contrast Media & Molecular Imaging·Sébastien de BournonvilleGreet Kerckhofs
Mar 5, 2020·Scientific Reports·Simone FerstlFranz Pfeiffer

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