Evaluation of cryoprotectant and cooling rate for sperm cryopreservation in the euryhaline fish medaka Oryzias latipes.

Cryobiology
Huiping YangTerrence R Tiersch

Abstract

Medaka Oryzias latipes is a well-recognized biomedical fish model because of advantageous features such as small body size, transparency of embryos, and established techniques for gene knockout and modification. The goal of this study was to evaluate two critical factors, cryoprotectant and cooling rate, for sperm cryopreservation in 0.25-ml French straws. The objectives were to: (1) evaluate the acute toxicity of methanol, 2-methoxyethanol (ME), dimethyl sulfoxide (Me(2)SO), N,N-dimethylacetamide (DMA), N,N-dimethyl formamide (DMF), and glycerol with concentrations of 5%, 10%, and 15% for 60min of incubation at 4°C; (2) evaluate cooling rates from 5 to 25°C/min for freezing and their interaction with cryoprotectants, and (3) test fertility of thawed sperm cryopreserved with selected cryoprotectants and associated cooling rates. Evaluation of cryoprotectant toxicity showed that methanol and ME (5% and 10%) did not change the sperm motility after 30min; Me(2)SO, DMA, and DMF (10% and 15%) and glycerol (5%, 10% and 15%) significantly decreased the motility of sperm within 1min after mixing. Based on these results, methanol and ME were selected as cryoprotectants (10%) to evaluate with different cooling rates (from 5 to 25°C/min) ...Continue Reading

Citations

Jan 26, 2012·Cryobiology·K A AlmansooriN M Jomha
Sep 3, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Terrence R TierschE Hu
May 28, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Huiping YangTerrence R Tiersch
Mar 29, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Terrence R TierschE Hu
Apr 6, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vahid ZadmajidIan Anthony Ernest Butts
May 12, 2019·Reproduction in Domestic Animals = Zuchthygiene·Daniella Aparecida de Jesus PaulaSilvana Marcussi
Jul 16, 2021·Frontiers in Physiology·Shaharior HossenKang Hee Kho

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