Evaluation of Cryptosporidium parvum oocyst recovery efficiencies from various filtration cartridges by electrochemiluminescence assays

Letters in Applied Microbiology
Y LeeV C W Tsang

Abstract

To evaluate four types of filtration cartridges for their capacities, efficiency for capture and release of Cryptosporidium parvum oocysts for detection. Filtration cartridges included in this evaluation were IDEXX Filta-Max, Gelman Envirochek HV, Corning CrypTest, and Filterite Sigma+. Various dosages of C. parvum oocysts were spiked into water samples with a wide range of turbidity (10-50 NTU). Electrochemiluminescence assays were employed to enumerate viable or total number of C. parvum oocysts in these eluates. Among the cartridges tested, Filta-Max consistently showed higher oocyst recovery efficiency, especially with large volume, highly turbid water samples. Filta-Max filter is the best performer because of its higher oocyst recovery efficiency. The overall sensitivities of various C. parvum oocyst detection assays in water samples can be improved if highly efficient oocyst recovery filtration cartridges such as Filta-Max are incorporated in sample preparation.

References

Oct 1, 1991·Applied and Environmental Microbiology·J S Hansen, J E Ongerth
Dec 1, 1991·Epidemiology and Infection·A J RichardsonR T Mayon-White
Sep 1, 1991·Applied and Environmental Microbiology·M W LeChevallierR G Lee
Aug 1, 1990·Journal of Medical Microbiology·P A ChapmanJ McLauchlin
Apr 1, 1987·Applied and Environmental Microbiology·J E Ongerth, H H Stibbs
Apr 1, 1987·Applied and Environmental Microbiology·C E MusialC P Gerba
Aug 1, 1995·Epidemiology and Infection·F AthertonD P Casemore
Mar 1, 1995·Letters in Applied Microbiology·J E Aldom, A H Chagla
Jul 21, 1994·The New England Journal of Medicine·W R Mac KenzieJ B Rose
Nov 1, 1995·Applied and Environmental Microbiology·D W JohnsonJ B Rose
Apr 1, 1996·Applied and Environmental Microbiology·K M Shepherd, A P Wyn-Jones
Jan 9, 1998·International Journal for Parasitology·G VeseyD A Veal
Oct 31, 1998·Applied and Environmental Microbiology·Z BukhariJ L Clancy
Jan 5, 2002·Molecular and Biochemical Parasitology·Xiaochuan FengGiovanni Widmer
Nov 27, 2002·Applied and Environmental Microbiology·Carol L DiGiorgioChristopher C Huitt

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Citations

Mar 24, 2012·Biomedical Microdevices·Majid Ebrahimi WarkianiHai-Qing Gong
Nov 4, 2011·The American Journal of Tropical Medicine and Hygiene·Ronalda S AraújoMaria Helena Matté
Apr 12, 2013·Rehabilitation Research and Practice·Grant L Iverson, Michael S Koehle
Jun 20, 2006·Environmental Research·P KaranisK Stojanova
Sep 20, 2008·Analytical and Bioanalytical Chemistry·Caroline PeskollerMichael Seidel

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