Evaluation of crystal quality of thin protein crystals based on the dynamical theory of X-ray diffraction

IUCrJ
Marina AbeMasaru Tachibana

Abstract

Knowledge of X-ray diffraction in macromolecular crystals is important for not only structural analysis of proteins but also diffraction physics. Dynamical diffraction provides evidence of perfect crystals. Until now, clear dynamical diffraction in protein crystals has only been observed in glucose isomerase crystals. We wondered whether there were other protein crystals with high quality that exhibit dynamical diffraction. Here we report the observation of dynamical diffraction in thin ferritin crystals by rocking-curve measurement and imaging techniques such as X-ray topography. It is generally known that in the case of thin crystals it is difficult to distinguish whether dynamical diffraction occurs from only rocking-curve profiles. Therefore, our results clarified that dynamical diffraction occurs in thin protein crystals because fringe contrasts similar to Pendellösung fringes were clearly observed in the X-ray topographic images. For macromolecular crystallography, it is hard to obtain large crystals because they are difficult to crystallize. For thin crystals, dynamical diffraction can be demonstrated by analysis of the equal-thickness fringes observed by X-ray topography.

References

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Mar 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ryo SuzukiMasaru Tachibana

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Citations

May 6, 2021·Acta Crystallographica. Section D, Structural Biology·Haruhiko KoizumiYoshihisa Suzuki

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Methods Mentioned

BETA
X-ray
dynamical diffraction

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