Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Walter C ProzialeckJoshua R Edwards


Cadmium (Cd) is a nephrotoxic environmental pollutant that causes insidious injury to the proximal tubule that results in severe polyuria and proteinuria. Cystatin C is a low molecular weight protein that is being evaluated as a serum and urinary biomarker for various types of ischemic and nephrotoxic renal injury. The objective of the present study was to determine if cystatin C might be a useful early biomarker of Cd nephrotoxicity. Male Sprague-Dawley rats were given daily injections of Cd for up to 12 weeks. At 3, 6, 9 and 12 weeks, urine samples were analyzed for cystatin C, protein, creatinine, β2 microglobulin and kidney injury molecule-1. The results showed that Cd caused a significant increase in the urinary excretion of cystatin C that occurred 3-4 weeks before the onset of polyuria and proteinuria. Serum levels of cystatin C were not altered by Cd. Immunolabeling studies showed that Cd caused the relocalization of cystatin C from the cytoplasm to the apical surface of the epithelial cells of the proximal tubule. The Cd-induced changes in cystatin C labelling paralleled those of the brush border transport protein, megalin, which has been implicated as a mediator of cystatin C uptake in the proximal tubule. These resul...Continue Reading


Nov 1, 1989·Toxicology and Applied Pharmacology·R A GoyerW Victery
Feb 1, 1996·Australian and New Zealand Journal of Ophthalmology·H MacleanA M Sandland
Jun 20, 1997·The Science of the Total Environment·A Bernard, C Hermans
Jun 2, 1998·Kidney International·C M Herak-KrambergerI Sabolić
Jun 22, 1999·Journal of Toxicology and Environmental Health. Part a·Z A ShaikhP Vestergaard
Sep 16, 1999·Toxicology and Applied Pharmacology·K ArsalaneA Bernard
Aug 24, 2001·American Journal of Obstetrics and Gynecology·A BökenkampM Kirschstein
Mar 21, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Lars Järup
Jul 26, 2002·The Journal of Biological Chemistry·Veronique BaillyJoseph V Bonventre
May 22, 2003·International Journal of Urology : Official Journal of the Japanese Urological Association·Teiichiro AoyagiMakoto Hata
May 6, 2004·American Journal of Physiology. Renal Physiology·R Bryan KlassenTimothy G Hammond
Dec 21, 2004·Clinical Biochemistry·Guido FillerAnders Grubb
Nov 3, 2005·Environmental Health Perspectives·Agneta AkessonStaffan Skerfving
May 13, 2006·The Journal of Pharmacology and Experimental Therapeutics·Natascha A WolffFrank Thévenod
Apr 28, 2007·Biochemical and Biophysical Research Communications·Ryohei KasedaAkihiko Saito
Aug 10, 2007·Kidney International·W C ProzialeckJ V Bonventre
Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Vishal S VaidyaJoseph V Bonventre
Apr 11, 2008·Critical Care Medicine·Chirag R Parikh, Prasad Devarajan
Apr 17, 2008·The Journal of Clinical Investigation·Takaharu IchimuraJoseph V Bonventre
May 30, 2008·Kidney International·Hua ZhouRobert A Star
Sep 13, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ajay P SharmaGuido Filler
Oct 2, 2008·The Journal of Toxicological Sciences·Jun Sekizawa
Mar 31, 2009·Toxicology and Applied Pharmacology·Joshua R Edwards, Walter C Prozialeck
Apr 14, 2009·Toxicology and Applied Pharmacology·Celia ByrneMary Beth Martin
Apr 18, 2009·Toxicology and Applied Pharmacology·Walter C ProzialeckJoseph V Bonventre
Apr 18, 2009·Toxicology and Applied Pharmacology·Pius Joseph
May 5, 2009·Toxicology and Applied Pharmacology·Lars Järup, Agneta Akesson
Jun 6, 2009·Pflügers Archiv : European journal of physiology·Erik Ilsø ChristensenRikke Nielsen
Aug 25, 2009·American Journal of Epidemiology·Ana Navas-AcienVirginia Weaver
Jan 29, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Walter C Prozialeck, Joshua R Edwards
Apr 16, 2010·Current Opinion in Nephrology and Hypertension·Lesley A StevensAndrew S Levey
Jun 1, 2010·Annals of Clinical Biochemistry·Chirag R ParikhPrasad Devarajan
Jun 17, 2010·Biomarkers in Medicine·Josef S OzerShashi Ramaiah
Jun 26, 2010·Environmental Health Perspectives·Patrizia GenaWilliam B Guggino
May 24, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Zhongheng ZhangNi Jin

❮ Previous
Next ❯


Dec 6, 2016·Biomarkers in Medicine·Hashem Nayeri, Nasim Babaknejad
Feb 1, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Frank ThévenodRalf Zarbock
Mar 25, 2017·European Journal of Nuclear Medicine and Molecular Imaging·Anna YordanovaHojjat Ahmadzadehfar
Aug 2, 2017·Journal of Biochemical and Molecular Toxicology·Ola Sayed Mohamed AliMamdouh Salah El-Din Zohni
Mar 15, 2018·Toxics·Soisungwan Satarug
Dec 30, 2017·Journal of Biochemical and Molecular Toxicology·Shereen Saeid Elshaer, Hend Mohamed Anwar
Sep 5, 2018·The Journal of the American Osteopathic Association·Walter C Prozialeck, Joshua R Edwards
Dec 14, 2018·Environmental Science and Pollution Research International·Shaukat AliMazhar Ulhaq
Jul 25, 2019·Antimicrobial Agents and Chemotherapy·Medha D JoshiBrooke Griffin
May 13, 2017·International Journal of Molecular Sciences·Sarah E Orr, Christy C Bridges
Aug 28, 2020·Environmental Science and Pollution Research International·Mona M TahaEman M El Tahlawy
Jan 13, 2021·Environmental Science and Pollution Research International·Rogelio Flores-RamírezLorena Díaz de León-Martínez
Aug 28, 2021·Children·Yuri Levin-SchwartzAlison P Sanders

❮ Previous
Next ❯

Methods Mentioned

enzyme-linked immunoabsorbent assay

Software Mentioned

Nikon Elements C
GraphPad Prism
Image J
Pro Plus

Related Concepts

Related Feeds