Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Walter C ProzialeckJoshua R Edwards

Abstract

Cadmium (Cd) is a nephrotoxic environmental pollutant that causes insidious injury to the proximal tubule that results in severe polyuria and proteinuria. Cystatin C is a low molecular weight protein that is being evaluated as a serum and urinary biomarker for various types of ischemic and nephrotoxic renal injury. The objective of the present study was to determine if cystatin C might be a useful early biomarker of Cd nephrotoxicity. Male Sprague-Dawley rats were given daily injections of Cd for up to 12 weeks. At 3, 6, 9 and 12 weeks, urine samples were analyzed for cystatin C, protein, creatinine, β2 microglobulin and kidney injury molecule-1. The results showed that Cd caused a significant increase in the urinary excretion of cystatin C that occurred 3-4 weeks before the onset of polyuria and proteinuria. Serum levels of cystatin C were not altered by Cd. Immunolabeling studies showed that Cd caused the relocalization of cystatin C from the cytoplasm to the apical surface of the epithelial cells of the proximal tubule. The Cd-induced changes in cystatin C labelling paralleled those of the brush border transport protein, megalin, which has been implicated as a mediator of cystatin C uptake in the proximal tubule. These resul...Continue Reading

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Citations

Dec 6, 2016·Biomarkers in Medicine·Hashem Nayeri, Nasim Babaknejad
Feb 1, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Frank ThévenodRalf Zarbock
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Jan 13, 2021·Environmental Science and Pollution Research International·Rogelio Flores-RamírezLorena Díaz de León-Martínez
Aug 28, 2021·Children·Yuri Levin-SchwartzAlison P Sanders

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Methods Mentioned

BETA
enzyme-linked immunoabsorbent assay

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Nikon Elements C
GraphPad Prism
MagPix xPONENT
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