Evaluation of cytokeratin-19 in breast cancer tissue samples: a comparison of automatic and manual evaluations of scanned tissue microarray cylinders

Biomedical Engineering Online
Cristina CallauCarlos López

Abstract

Digital image (DI) analysis avoids visual subjectivity in interpreting immunohistochemical stains and provides more reproducible results. An automated procedure consisting of two variant methods for quantifying the cytokeratin-19 (CK19) marker in breast cancer tissues is presented. The first method (A) excludes the holes inside selected CK19 stained areas, and the second (B) includes them. 93 DIs scanned from complete cylinders of tissue microarrays were evaluated visually by two pathologists and by the automated procedures. There was good concordance between the two automated methods, both of which tended to identify a smaller CK19-positive area than did the pathologists. The results obtained with method B were more similar to those of the pathologists; probably because it takes into account the entire positive tumoural area, including the holes. However, the pathologists overestimated the positive area of CK19. Further studies are needed to confirm the utility of this automated procedure in prognostic studies.

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Citations

Sep 4, 2015·Biomedical Engineering Online·Franscisco M OrtuñoIgnacio Rojas
Sep 30, 2016·Journal of Microscopy·L RoszkowiakT Markiewicz
May 2, 2019·Journal of Cellular Physiology·Masoumeh MehrpouyaMorteza Oladnabi
Jun 8, 2019·International Journal of Cancer. Journal International Du Cancer·Pablo Vicente-MunueraLuis M Escudero

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Methods Mentioned

BETA
chromosomal aberrations
biopsy
biopsies

Software Mentioned

VISILAB
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