Evaluation of cytokine production from peripheral blood mononuclear cells of type 1 diabetic patients

Annals of the New York Academy of Sciences
Maria Cristina Foss-FreitasMilton C Foss

Abstract

This study aims to evaluate the production of cytokines, tumor necrosis factor (TNF), and interleukin 10 (IL-10) in peripheral blood mononuclear cells (PBMCs) from type 1 diabetic (T1D) patients by means of intracellular staining, flow cytometry, and ELISA and to correlate it with inadequate (IN) and adequate (A) metabolic controls. We studied 28 patients with T1D and 20 healthy individuals (C) paired by sex and age. T1D patients were divided in patients with IN and A metabolic control. PBMC cultures were stimulated with LPS to evaluate TNF or were stimulated with PMA/ionomycin or concanavalin A to evaluate IL-10. The TNF levels in supernatant of stimulated cultures, evaluated by ELISA, of diabetic patients were similar to those of healthy individuals, although the percentage of CD 33(+) cells that were positive for TNF was higher in the T1D IN group compared to the T1D A group (P= 0.01). Similarly, the IL-10 levels evaluated by ELISA in stimulated cultures of T1D patients were not different from those in the control group; moreover, the percentage of CD3(+) cells positive for intracellular IL-10 were higher in the T1D IN group compared to C groups (P= 0.007). The increased levels of cytokines in T1D IN diabetic patients, with ...Continue Reading

References

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Citations

Jun 22, 2012·Journal of Cellular Physiology·Dana L CatalfamoShannon M Wallet
Jun 10, 2010·Folia Histochemica Et Cytobiologica·Włodzimierz ŁuczyńskiArtur Bossowski
Jan 30, 2018·Frontiers in Molecular Biosciences·Partho SenMatej Orešič
Dec 1, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E LachmandasJ A van Diepen

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