Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test

PLoS Neglected Tropical Diseases
Warllem Junio OliveiraStefan Michael Geiger

Abstract

In some tropical countries, such as Brazil, schistosomiasis control programs have led to a significant reduction in the prevalence and parasite burden of endemic populations. In this setting, the Kato-Katz technique, as the standard diagnostic method for the diagnosis of Schistosoma mansoni infections, which involves the analysis of two slides from one fecal sample, loses its sensitivity. As a result, a significant number of infected individuals are not detected. The objective of this study was to perform extensive parasitological testing of up to three fecal samples and include a rapid urine test (POC-CCA) in a moderate prevalence area in Northern Minas Gerais, Brazil, and evaluate the performance of each test separately and in combination. A total of 254 individuals were examined with variants of the standard Kato-Katz technique (up to18 Kato-Katz slides prepared from three fecal samples), a modified Helmintex (30 g of feces), the saline gradient (500 mg of feces), and the POC-CCA methods. We established a reference standard taking into consideration all the positive results in any of the parasitological exams. Evaluation of the parasite burden by two Kato-Katz slides confirmed that most of the individuals harbored a light in...Continue Reading

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Citations

Nov 9, 2018·PLoS Neglected Tropical Diseases·José M Peralta, Marta G Cavalcanti
Jan 10, 2019·International Journal of Environmental Research and Public Health·Linhan LiQingwu Jiang
May 31, 2019·Infectious Diseases of Poverty·Sergei Rodrigo Magalhães de SousaMartin Johannes Enk
Apr 4, 2019·Memórias do Instituto Oswaldo Cruz·Vanessa Silva-MoraesPaulo Marcos Zech Coelho
Mar 11, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Guilherme Vaz de Melo TrindadeJosé Roberto Lambertucci
Mar 23, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Enas A El Saftawy
Apr 23, 2021·Journal of Theoretical Biology·Thomas N VilchesHyun Mo Yang
Jun 19, 2021·Frontiers in Medicine·Silvana Júnia RorizJosé Roberto Lambertucci
Jul 7, 2021·PLoS Neglected Tropical Diseases·Marie-Laure GillardiePierre Flori

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Software Mentioned

Open Epi
GraphPad Prism
CCA

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