Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs

Vaccines
Stefano PetriniMaura Ferrari

Abstract

In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) a...Continue Reading

References

Mar 24, 1989·Cell·A Bachmair, A Varshavsky
Jul 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·I J YoonT W Molitor
Nov 5, 1997·Nature Biotechnology·J B Ulmer
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RoveroG Forni
Dec 6, 2002·The Journal of General Virology·R A PontarolloS van Drunen Littel-van den Hurk
Jan 18, 2003·Vaccine·L A BabiukS van Drunen Littel-van den Hurk
Oct 28, 2004·Veterinary Immunology and Immunopathology·O J Lopez, F A Osorio
Oct 11, 2005·Veterinary Immunology and Immunopathology·Paolo BorghettiPaolo Martelli
Nov 13, 2009·Comparative Immunology, Microbiology and Infectious Diseases·S PetriniM Ferrari
Jan 13, 2010·Vaccine·Joshua S EllingsonKay S Faaberg
Jul 28, 2010·Virus Research·Lei Zhou, Hanchun Yang

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Methods Mentioned

BETA
PCR
confocal microscopy
transfection
ELISA
Enzyme-Linked Immunosorbent Assay
FCS
flow cytometry

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