PMID: 11332034May 3, 2001Paper

Evaluation of different photosensitizers for use in photochemical gene transfection

Photochemistry and Photobiology
Lina PrasmickaiteK Berg

Abstract

Many potentially therapeutic macromolecules, e.g. transgenes used in gene therapy, are taken into the cells by endocytosis, and have to be liberated from endocytic vesicles in order to express a therapeutic function. To achieve this we have developed a new technology, named photochemical internalization (PCI), based on photochemical reactions inducing rupture of endocytic vesicles. The aim of this study was to clarify which properties of photosensitizers are important for obtaining the PCI effect improving gene transfection. The photochemical effect on transfection of human melanoma THX cells has been studied employing photosensitizers with different physicochemical properties and using two gene delivery vectors: the cationic polypeptide polylysine and the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). Photochemical treatment by photosensitizers that do not localize in endocytic vesicles (tetra[3-hydroxyphenyl]porphyrin and 5-aminolevulinic acid-induced protoporphyrin IX) do not stimulate transfection, irrespective of the gene delivery vector. In contrast, photosensitizers localized in endocytic vesicles stimulate polylysine-mediated transfection, and amphiphilic photosensitizers (disulfonated aluminium phthal...Continue Reading

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Citations

Jul 24, 2002·Archives of Pharmacal Research·Chong-Kook Kim, Soo-Jeong Lim
Jan 7, 2004·Advanced Drug Delivery Reviews·Anders HøgsetKristian Berg
May 22, 2002·Biochimica Et Biophysica Acta·Lina PrasmickaiteKristian Berg
Mar 1, 2012·Accounts of Chemical Research·Yuka Matsushita-Ishiodori, Takashi Ohtsuki
Nov 22, 2005·Nature Materials·Nobuhiro NishiyamaKazunori Kataoka
Oct 19, 2005·Gene Therapy·S DinçerE Pişkin
Oct 19, 2011·Nucleic Acid Therapeutics·Sigurd L BøeEivind Hovig
Jan 20, 2006·Photochemistry and Photobiology·Lina PrasmickaiteKristian Berg
Sep 13, 2013·Therapeutic Delivery·Sigurd Leinæs Bøe, Eivind Hovig
Feb 26, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Roman LehnerPatrick Hunziker
Mar 7, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ying Waeckerle-MenPål Johansen
Apr 23, 2009·Advanced Drug Delivery Reviews·Nobuhiro NishiyamaKazunori Kataoka
Mar 1, 2007·Photodiagnosis and Photodynamic Therapy·Asta Juzeniene, Johan Moan
Jul 4, 2013·Photochemistry and Photobiology·Marie VikdalKristian Berg
Sep 1, 2004·Expert Opinion on Biological Therapy·Lina PrasmickaiteKristian Berg
Jul 6, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pål Kristian SelboKristian Berg
Feb 22, 2011·Biochimica Et Biophysica Acta·Mattia MarzoratiMartina Vermathen
Jun 17, 2008·IEEE Transactions on Nanobioscience·C P YaoG Huettmann
Jul 3, 2013·Nature Communications·Yu-Hsien HungWei Yuan Yang
Apr 2, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Andreas DietzeK Berg
Mar 7, 2006·The Journal of Gene Medicine·Birgit Ø EngesaeterGunhild M Maelandsmo
Jul 21, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kristian BergAnders Høgset
Oct 28, 2004·Journal of Drug Targeting·Julia KloecknerErnst Wagner
Dec 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kristian BergAnders Høgset
Feb 3, 2021·Journal of Pharmaceutical Sciences·Melissa Siaw Han LimEiji Matsuura
Sep 9, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Julia KloecknerErnst Wagner
Nov 29, 2013·Journal of the American Chemical Society·Huabing ChenKazunori Kataoka
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma

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