PMID: 9533077Apr 9, 1998Paper

Evaluation of different procedures for processing avian air sac lavage fluid

Avian Diseases
R CrespoD B Hunter

Abstract

Air sac lavage has become a routine diagnostic tool used in pet birds; however, cytologic techniques for collection and handling of avian cells have not been reported. In this study, directed endoscopy was used to obtain the air sac washes. Different factors were compared that might affect final preservation of cell morphology, such as centrifugation speed, anticoagulant, storage temperature, time to processing, addition of glutaraldehyde to the sample, and addition of protein. The goal was to find the processing technique best suited for the evaluation of cells from air sac washes. The use of an anticoagulant did not influence cell quality. Optimal cell morphology was achieved when the sample was centrifuged at a speed of 1000 rpm (89.4 X g) and when prepared in less than an hour from the time of collection. Low temperature (5 C) tended to preserve cell quality better. The addition of protein (fetal bovine serum) helped to preserve cell quality and was of value if the sample storage time was greater than 30 min. The addition of glutaraldehyde resulted in a significant reduction in cell quality.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.