Evaluation of DNA barcoding and identification of new haplomorphs in Canadian deerflies and horseflies

Medical and Veterinary Entomology
A CywinskaF F Hunter

Abstract

This paper reports the first tests of the suitability of the standardized mitochondrial cytochrome c oxidase subunit I (COI) barcoding system for the identification of Canadian deerflies and horseflies. Two additional mitochondrial molecular markers were used to determine whether unambiguous species recognition in tabanids can be achieved. Our 332 Canadian tabanid samples yielded 650 sequences from five genera and 42 species. Standard COI barcodes demonstrated a strong A + T bias (mean 68.1%), especially at third codon positions (mean 93.0%). Our preliminary test of this system showed that the standard COI barcode worked well for Canadian Tabanidae: the target DNA can be easily recovered from small amounts of insect tissue and aligned for all tabanid taxa. Each tabanid species possessed distinctive sets of COI haplotypes which discriminated well among species. Average conspecific Kimura two-parameter (K2P) divergence (0.49%) was 12 times lower than the average divergence within species. Both the neighbour-joining and the Bayesian methods produced trees with identical monophyletic species groups. Two species, Chrysops dawsoni Philip and Chrysops montanus Osten Sacken (Diptera: Tabanidae), showed relatively deep intraspecific seq...Continue Reading

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Citations

Oct 31, 2012·Medical and Veterinary Entomology·M AnderJ Chirico
Oct 30, 2016·Acta Tropica·Carolina Torres-GutierrezMaria Anice Mureb Sallum
Aug 28, 2015·Memórias do Instituto Oswaldo Cruz·Richard Hoyos-LópezJuan Carlos Gallego-Gómez
Oct 3, 2020·Parasites & Vectors·Mohamed Lamine KeitaOleg Mediannikov
Aug 31, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yi-Tian FuYu Zhao

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