Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants

Mutation Research
Tomás GichnerJirina Száková

Abstract

Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 microM to 200 microM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 microM Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobacco with 2.4 mM Pb2+, the accumulation of the heavy metal is 40-fold higher in the roots than in the above-ground biomass. Low Pb2+ accumulation in the above-ground parts may explain the lower levels or the absence of Pb2+-induced DNA d...Continue Reading

References

Apr 17, 1998·Environmental and Molecular Mutagenesis·H SteinkellnerS Knasmüller
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Oct 15, 2005·Ecotoxicology and Environmental Safety·Tomás GichnerKaterina Demnerová

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Citations

May 26, 2012·Chemical Research in Toxicology·Cristina MonteiroMaria Celeste Dias
Oct 26, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Sofia CorreiaOlinda Pinto-Carnide
Sep 10, 2014·Ecotoxicology and Environmental Safety·Karolina IzbiańskaJoanna Deckert
Nov 8, 2013·Drug and Chemical Toxicology·Anshita R Purohit, Mandava Venkatesh Rao
Jan 20, 2016·Environmental Science and Pollution Research International·Mumtaz KhanShui Jin Zhu
Nov 24, 2012·Plant Physiology and Biochemistry : PPB·E RodriguezConceição Santos
Sep 24, 2015·Environmental Science and Pollution Research International·C LanierA Deram
Jan 7, 2016·Environmental Science and Pollution Research International·Luiz Cláudio Cardozo ChavesViviane Souza do Amaral
Jan 26, 2011·Mutation Research·Lai-Jun ZhangTian-Ke Li
Jan 26, 2011·Comptes rendus biologies·Dharman DhakshanamoorthyA L A Chidambaram
Sep 4, 2015·Environmental Pollution·Caroline LanierAnnabelle Deram
Nov 25, 2014·Bulletin of Environmental Contamination and Toxicology·Daniela S Garanzini, Mirta L Menone
Jun 8, 2011·Mutation Research·Erico R CarmonaRicard Marcos
Sep 17, 2011·Mutation Research·Bertrand PourrutEric Pinelli
May 14, 2016·Nanoscale Research Letters·Jieun Kim, Jong Bum Lee
Oct 13, 2019·Environmental Science and Pollution Research International·Eleazar RodriguezConceição Santos
Mar 29, 2020·International Journal of Environmental Research and Public Health·Amit KumarKrishna K Yadav
Mar 27, 2018·3 Biotech·Noha S Khalifa, Mohammed N Hasaneen
Mar 28, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ricardo Ortiz-LuevanoLenin Sánchez-Calderón
Jun 15, 2011·Chemical Research in Toxicology·Eleazar RodriguezConceição Santos
Nov 7, 2021·The Plant Journal : for Cell and Molecular Biology·José Antonio Pedroza-GarciaLieven De Veylder

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