Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Journal of Occupational Health
Muhammad KhisroonSyed Basit Rasheed

Abstract

The importance of X-rays as a diagnostic medical tool cannot be denied. However, continuous exposure to X-rays can cause DNA damage. This study aimed to use the comet assay technique to investigate the level of DNA damage in lymphocytes due to X-rays in occupationally exposed personnel. Blood samples were collected from 74 exposed and 70 control subjects for analysis. A total of 100 randomly captured cells from each slide were examined using an epifluorescent microscope. The comets were analyzed by a visual scoring method according to comet tail length. The results indicated a significant increase (p<0.05) in DNA damage in X-rays technicians (129.8 ± 17.2) as compared with the control group (53.0 ± 25.0). A significant increase (p<0.02) in DNA damage was also observed with an increase in exposure duration of technicians because of their service length. The present study suggests that the exposed radiology personnel should carefully comply with radiation protection procedures such as wearing of lead apron during diagnostic procedures and minimize radiation exposure where possible to avoid potential genotoxic effects due to X-rays.

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Citations

Sep 25, 2018·International Journal of Occupational and Environmental Health·Muhammad Khisroon Ahmadullah
Feb 15, 2019·Journal of Animal Physiology and Animal Nutrition·Heba S A TahaMahmoud Alagawany
Jun 28, 2019·Arhiv za higijenu rada i toksikologiju·Dauren BotbayevSabrina Angelini
Aug 10, 2020·Journal of Occupational and Environmental Medicine·Muhammad KhisroonJaveed Farooqi
Nov 18, 2018·Bulletin of Environmental Contamination and Toxicology·Muhammad Iftikhar KhanMuhammad Khisroon
Jan 13, 2018·Bulletin of Environmental Contamination and Toxicology·Muhammad SirajGhani Rahman
Sep 6, 2020·Journal of Occupational and Environmental Medicine·Muhammad KhisroonJaveed Farooqi
Jul 18, 2020·Biological Trace Element Research·Muhammad KhisroonAbid Ullah
Apr 19, 2021·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Katia Soares da PoçaMarcia Sarpa
Jul 25, 2021·International Journal of Molecular Sciences·Clémence BaudinMaria Grazia Andreassi

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