Evaluation of DNA damage induced by atrazine and atrazine-based herbicide in human lymphocytes in vitro using a comet and DNA diffusion assay

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Davor ZeljezicPetra Perkovic

Abstract

Atrazine is one of the most widely used herbicides in the world. When applied, it is not used as a pure active ingredient but in the form of commercial formulations. Besides atrazine, these formulations contain other substances that might represent a risk to human health due to their mutual interactions. We evaluated the genotoxicity, apoptosis and necrosis induction of atrazine as an active ingredient, the commercial formulation Gesaprim, and a Gesaprim adjuvant mixture without atrazine by comet and DNA diffusion assay, respectively. Human lymphocytes were treated for 0.5, 1, 3, 5, and 8 h with 0.047 microg/ml, 0.47 microg/ml, 4.7 microg/ml of substances tested both in the presence and in the absence of an exogenous metabolic activator. Atrazine did not appear to be genotoxic or to be capable of inducing apoptosis or necrosis. Unlike atrazine, Gesaprime and the adjuvant mixture increased DNA damage in lymphocytes. After 5 h of treatment, it also increased the number of apoptotic cells. Metabolic activation additionally enhanced the DNA-damaging potential of Gesaprim and the adjuvant mixture but did not affect atrazine genotoxicity. Thus, both assay endpoints differed significantly for the active ingredient and formulation. To ...Continue Reading

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis