Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay

Carcinogenesis
J M BarretB T Hill

Abstract

A recently derived in vitro chemiluminescence assay (Salles et al. [1995] Anal. Biochem., 232, 37-42) has been used to investigate the effects of a panel of twenty-two anticancer drugs and certain antibiotics on the excision repair activity of cell-free extracts from the human cell line, HeLa. This methodology, termed the 3D (Damaged DNA Detection) assay, based on the in vitro excision repair assay previously developed (Wood et al. [1988] Cell, 53, 97-106) has provided data indicating definite in vitro inhibition of DNA repair by actinomycin D, aphidicolin, doxorubicin, distamycin A and mithramycin A. This assay therefore offers the potential for identifying agents with the ability to inhibit DNA repair.

Citations

Jul 6, 2000·FEBS Letters·S PlanchaisC Bergounioux
Oct 20, 2001·Experimental Cell Research·A L BlajeskiS H Kaufmann
May 2, 2018·Pharmaceutics·Tomasz M Karpiński, Artur Adamczak
Jun 27, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Atieh YaghoubiWilliam C Cho

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