Evaluation of drug effects on cerebral blood flow and glucose uptake in un-anesthetized and un-stimulated rats: application of free-moving apparatus enabling to keep rats free during PET/SPECT tracer injection and uptake

Nuclear Medicine Communications
Taku SugitaYasuhiro Magata

Abstract

The purpose of this study is the development of novel fluorine-18-fluorodeoxyglucose (F-FDG)-PET and Tc-hexamethylpropylene amine oxime (HMPAO) SPECT methods with free-moving apparatus on conscious rats to investigate brain activity without the effects of anesthesia and tactual stimulation. We also assessed the sensitivity of the experimental system by an intervention study using fluoxetine as a reference drug. A catheter was inserted into the femoral vein and connected to a free-moving cannula system. After fluoxetine administration, the rats were given an injection of F-FDG or Tc-HMPAO via the intravenous cannula and released into a free-moving cage. After the tracer was trapped in the brain, the rats were anesthetized and scanned with PET or SPECT scanners. Then a volume of interest analysis and statistical parametric mapping were performed. We could inject the tracer without touching the rats, while keeping them conscious until the tracers were distributed and trapped in the brain using the developed system. The effects of fluoxetine on glucose uptake and cerebral blood flow were perceptively detected by volume of interest and statistical parametric mapping analysis. We successfully developed free-moving F-FDG-PET and Tc-HM...Continue Reading

References

Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J D'AmatoS H Snyder
Sep 27, 2003·Journal of Neuroscience Methods·Petra SchweinhardtJesper L R Andersson
Aug 3, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bojana StefanovicAfonso C Silva
Mar 17, 2007·The Cerebellum·Susanne M Morton, Amy J Bastian
Feb 25, 2009·The International Journal of Neuropsychopharmacology·Takeaki SaijoTetsuya Suhara
Jun 18, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Hiroshi MizumaHirotaka Onoe
Aug 30, 2014·Pain Practice : the Official Journal of World Institute of Pain·Paula Pascoal-FariaFelipe Fregni
Sep 10, 2014·TheScientificWorldJournal·Takashi TemmaHidehiro Iida
Nov 12, 2014·Brain Connectivity·Chenxuan LiChristopher P Pawela
Dec 4, 2014·Metabolism: Clinical and Experimental·Richard Wurtman
Jan 22, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Manouchehr S VafaeePaul Cumming
Jul 16, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Stefano TarantiniPeter Toth
Sep 24, 2015·Nuclear Medicine and Molecular Imaging·Sonya Youngju ParkYong An Chung
Dec 23, 2015·Nuklearmedizin. Nuclear Medicine·Friedrich WelzPhilipp Ritt
Jan 23, 2016·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Jaakko PaasonenOlli Gröhn
Feb 9, 2016·Nature Methods·Xin YuAlan P Koretsky
Mar 15, 2016·Current Alzheimer Research·Matthias Brendel Alzheimer's Disease Neuroimaging Initiative
Apr 8, 2016·Journal of Neurophysiology·Pei-Ching ChangA Vania Apkarian
Apr 12, 2016·Frontiers in Pharmacology·Lieven D DeclercqGuy Bormans
Apr 26, 2016·Psychiatry Research. Neuroimaging·Alexander DoruyterJames M Warwick
Jan 8, 2017·Nuclear Medicine and Biology·Chie SuzukiYasuhiro Magata

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imaging techniques
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SPM
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STAT

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