Evaluation of dynamic weight bearing for measuring nonevoked inflammatory hyperalgesia in mice

Nursing Research
Mari A GriffioenCynthia L Renn

Abstract

Animal models in pain research have suggested that inclusion of both evoked and nonevoked behavioral measures is needed to better reflect the human pain experience. Individuals with chronic pain are known to experience spontaneous pain, in addition to pain after exposure to an external stimulus. Recently, the dynamic weight bearing (DWB) apparatus was developed to assess for nonevoked hyperalgesia by capturing weight bearing and surface distribution in the paws of mice after acute inflammation. The aim of this study was to evaluate the DWB test as a measure of nonevoked hyperalgesia. The experimental group received an intraplantar injection in the left hind paw of the inflammatory agent--complete Freund's adjuvant (CFA)--whereas the vehicle control group received a saline injection and the naive control group had no treatment. Calipers and a plethysmometer were used to verify inflammation and the hot-plate test was used as a measure for stimulus-evoked hyperalgesia. Data were collected at baseline; 3 hours; and 1, 3, and 7 days after injection. Mice injected with CFA showed a statistically significant higher mean paw thickness and volume displacement compared with the vehicle and naive control groups. In the hot-plate testing, ...Continue Reading

Citations

Aug 23, 2020·Current Osteoporosis Reports·Luke G McVeighMelissa A Kacena
Sep 22, 2017·Frontiers in Molecular Neuroscience·Jennifer R DeuisIrina Vetter
Oct 8, 2020·Animal Models and Experimental Medicine·Bharata Regmi, Manoj K Shah
Nov 25, 2018·Toxicon : Official Journal of the International Society on Toxinology·Nicole BlanshanHollis E Krug
May 14, 2021·European Journal of Pharmacology·Moanna VillaluzAndrew MacKenzie
Oct 6, 2021·Nature Communications·Rasheen PowellArin Bhattacharjee

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