Evaluation of early healing events around mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffold. An experimental study in Wistar rats.

Oral and Maxillofacial Surgery
M AlhagFergal J O'Brien

Abstract

Tissue engineering using cell-seeded biodegradable scaffolds offers a new bone regenerative approach that might circumvent many of the limitations of current therapeutic modalities. The aim of this experiment was to study the early healing events around mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffolds. The 5-mm critical size defects were created in the calvarial bones of 41 Wistar rats. The defects were either left empty to serve as controls (n = 11), filled with cell-free scaffolds (n = 12), cell-seeded scaffolds that were maintained in standard culture medium (n = 9), or cell-seeded scaffolds that were maintained in osteoinductive factor-supplemented medium (n = 9). The animals were sacrificed at 7 days after surgery, and specimens were prepared for histological analysis. Early healing events such as host cell penetration, blood vessel in-growth, and scaffold integration were observed. The degree of inflammatory cell infiltrate was assessed. While defects in the control group healed with a thin fibrous tissue, the collagen-glycosaminoglycan scaffold in the test groups preserved the three-dimensional form of the defects. After 7 days in vivo, the scaffold maintained its integrity and appeared populated with ho...Continue Reading

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Citations

Aug 1, 2013·Microvascular Research·Andreas KampmannFrank Tavassol
Apr 4, 2015·Regenerative Medicine·Thiago de Santana SantosAdalberto Luiz Rosa
Dec 6, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Erica G TierneyFergal J O'Brien
Mar 31, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elaine QuinlanAdolfo López-Noriega
Mar 30, 2013·Organogenesis·Erica G TierneyFergal J O'Brien
Dec 8, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elaine QuinlanFergal J O'Brien
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Feb 22, 2013·Materials·Amos MatsikoFergal J O'Brien
Nov 19, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Lucas Fabricio Bahia NogueiraAna Paula Ramos

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