Evaluation of endothelial and inducible nitric oxide synthase mRNA expression in the lung of broiler chickens with developmental pulmonary hypertension due to cold stress

British Poultry Science
Masood TeshfamHossein Hassanpour

Abstract

To clarify the effect of cold-induced pulmonary hypertension on endothelial and inducible nitric oxide synthase (eNOS and iNOS) mRNA expression in the lung of broiler chickens, semi quantitative reverse transcription-PCR was performed on total RNAs isolated from lungs of the broiler chickens exposed to 5 weeks of cold stress. The eNOS gene was expressed increasingly with the increasing age during the rearing period. Comparing the treatment group with its related control group eNOS was expressed significantly only at d 21. Expression of iNOS mRNA also increased in both control and treatment groups with increasing age until d 28 and then decreased at d 35 and 42. Comparing the treatment group with its control group, iNOS mRNA level was significantly higher at 21 d of age in the cold-exposed chickens. It was concluded that, although cold exposure could significantly increase eNOS and iNOS gene expression, cold-induced pulmonary hypertension is not associated with significant variations of eNOS and iNOS expression in the lungs of broiler chickens.

References

Apr 1, 1992·Poultry Science·J A Mench
Jun 1, 1994·The American Journal of Physiology·W R TraceyR A Johns
Nov 1, 1994·The American Journal of Physiology·L P McQuillanS Kourembanas
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Dec 1, 1994·The American Journal of Physiology·C XueR A Johns
Jan 1, 1995·Annual Review of Physiology·O W Griffith, D J Stuehr
Aug 1, 1995·American Journal of Respiratory Cell and Molecular Biology·P W ShaulR A Star
Oct 1, 1993·American Journal of Respiratory Cell and Molecular Biology·L KobzikJ S Stamler
Oct 1, 1994·Journal of the American Dietetic Association
Jun 1, 1994·Poultry Science·D Zaviezo, N Dale
Mar 1, 1996·The American Journal of Physiology·T C Resta, B R Walker
Jul 1, 1996·Journal of Applied Physiology·T KizakiH Ohno
Feb 1, 1997·The American Journal of Physiology·T C RestaB R Walker
Feb 25, 1998·The Journal of Thoracic and Cardiovascular Surgery·J E ScarboroughR M Ungerleider
Mar 5, 1998·The American Journal of Physiology·L A PalmerR A Johns
Feb 10, 1999·The American Journal of Physiology·R C TylerI F McMurtry
Aug 23, 2001·International Immunopharmacology·J W Coleman
Sep 12, 2002·Nitric Oxide : Biology and Chemistry·Huige LiUlrich Förstermann

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Citations

Sep 27, 2013·British Poultry Science·H HassanpourS H Bahadoran
May 13, 2014·Nitric Oxide : Biology and Chemistry·Wenwu SunZhuang Ma
Oct 30, 2010·Research in Veterinary Science·Hossein HassanpourBehzad Heydaripoor
Jul 25, 2015·International Journal of Biometeorology·Vijay Pratap YadavMihir Sarkar
Dec 17, 2015·Journal of Animal Physiology and Animal Nutrition·Y B Jiang, Y R Yang
May 20, 2014·Journal of Biomedical Materials Research. Part a·Fatemeh KabirianBehrouz Zandieh-Doulabi
Jul 9, 2009·Acta Veterinaria Hungarica·Hossein HassanpourHamed Zarei
Sep 18, 2014·Animal : an International Journal of Animal Bioscience·H HassanpourA Mohebbi
Nov 13, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Krishna R HamalGisela F Erf
Jun 20, 2019·British Poultry Science·H HassanpourS Madreseh

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Methods Mentioned

BETA
electrophoresis
reverse-transcription PCR
PCR

Software Mentioned

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