PMID: 2095632Mar 1, 1990Paper

Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera

Revista Do Instituto De Medicina Tropical De São Paulo
J Ivo-Dos-SantosB Galvão-Castro

Abstract

Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.

References

May 23, 1986·MMWR. Morbidity and Mortality Weekly Report·UNKNOWN Centers for Disease Control (CDC)
Jun 1, 1986·American Journal of Clinical Pathology·S L Sivak, G P Wormser
May 20, 1987·Journal of Immunological Methods·J Ivo dos SantosM S Pereira
May 15, 1987·JAMA : the Journal of the American Medical Association·B G CastroF O Cruz

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