Evaluation of enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infections

Veterinary Parasitology
Xiaohong HuangI Igarashi

Abstract

Two enzyme-linked immunosorbent assays (ELISA) with recombinant protein as antigens were evaluated by comparison with the indirect fluorescent antibody tests (IFAT) for the detection of specific antibodies to Babesia caballi and Babesia equi, respectively in 380 sera from experimentally infected, uninfected, and field horses. The high concordances of 92.4% (351/380) and 98.2% (373/380) between ELISA and IFAT for B. caballi and B. equi, respectively suggest that ELISA, especially for B. equi infection, could be alternative to the corresponding IFAT for serodiagnoses of equine piroplasmosis, although some improvements are required in ELISA for B. caballi.

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Citations

Mar 19, 2013·Parasitology International·Seung Won KangKi Yoon Chang
Mar 6, 2012·Parasitology International·Mohamad Alaa TerkawiIkuo Igarashi
Mar 25, 2011·Veterinary Parasitology·Min-Goo SeoDongmi Kwak
Oct 9, 2015·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Márcia Mariza Gomes JusiRosangela Zacarias Machado

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