Evaluation of Epic® label-free technology to quantify functional recombinant hemagglutinin

Biological Procedures Online
Lianlian Jiang, Maryna C Eichelberger

Abstract

Alternative methods are being sought to measure the potency of influenza vaccines. Label-free technologies that do not require the use of hemagglutinin (HA)-specific antisera are particularly attractive as the preparation of antiserum delays availability of potency reagents. The objective of these experiments was to evaluate the use of a Corning Epic® label-free method to quantify functional influenza hemagglutinin in rHA preparations. The method was optimized to quantify recombinant HA (rHA) of B/Brisbane/60/2008 (B/BR/08). Fetuin was immobilized onto plates and the change in wavelength of refracted light measured using an Enspire (Perkin Elmer) instrument. The change in wavelength measured in response to addition of rHA of B/BR/08 was proportional to its concentration and was optimal in the presence of native rHA conformations. However, the assay was strain-dependent and did not correlate with HAU measured using turkey red blood cells. The Corning Epic® label-free method is suitable for quantifying the native forms of rHA for B/BR/08 and A/Brisbane/59/2007 (H1N1) and A/Hangxhou/3/2013 (H7N9). This method is a useful tool for research purposes but further investigation is needed to identify suitable glycoproteins to use as lig...Continue Reading

References

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Aug 5, 2014·Influenza and Other Respiratory Viruses·Falko SchmeisserJerry P Weir

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Citations

Oct 28, 2015·Nature Reviews. Neurology·Ralf BaronMichael Weller

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Methods Mentioned

BETA
biosensing
surface plasmon resonance
chips
ELISAs
ELISA

Software Mentioned

Epic®

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