Evaluation of ex vivo expanded human NK cells on antileukemia activity in SCID-beige mice

Leukemia
F GuimarãesM Sirac Dilber

Abstract

The possibility of using natural killer (NK) cells in treatment of human hematological malignancies has increased in recent years. One factor contributing to this is the introduction of new methods for ex vivo generation of enriched populations of clinical grade NK cells. The objective of the present study was to evaluate the safety and efficacy of human ex vivo expanded clinical grade NK cells against K562 leukemia cells in severe combined immunodeficiency disease (SCID)-beige mice. Irradiated SCID-beige mice were injected intravenously (i.v.) with K562 leukemia cells. Following leukemia cell injection, mice were injected with ex vivo expanded human NK cells. NK cells were followed in vivo and mice monitored for survival from leukemia. Administration of these ex vivo expanded clinical grade NK cells was safe and prevented leukemia development. In conclusion, these results imply possibilities for the use of this NK cell preparation in treatment trials of human hematological malignancies and possibly other forms of cancer.

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Citations

Mar 5, 2008·Cancer Immunology, Immunotherapy : CII·Karl-Johan MalmbergHans-Gustaf Ljunggren
May 1, 2009·Immunotherapy·Lisbeth Barkholt, Marco Bregni
Nov 14, 2013·Current Opinion in Organ Transplantation·Seigo NishidaAndreas G Tzakis
Jun 5, 2018·Hematological Oncology·Alexandra JungeThomas Pabst

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